We narrowed to 16,162 results for: grna

1. sgRNA.SFFV.tBFP

   Plasmid

   #169940

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

   Depositor

   Benjamin Ebert

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_EZH2gRNA14

   Plasmid

   #199586

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGRNA3e

   Plasmid

   #169783

   Purpose

   Insert one gRNA

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGRNA2

   Plasmid

   #169779

   Purpose

   Insert one gRNA

   Depositor

   Sang-Gyu Kim

   Article

   Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. px458_2A_GFP_sgRNA_TIA1

   Plasmid

   #106097

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA TIA1
   

   Use

   CRISPR

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. STAGR_gRNAScaffold_hU6

   Plasmid

   #102840

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Promoter

   hU6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA_GFP-T2

   Plasmid

   #41820

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pColE1_sgRNA_pos8_AmpR

   Plasmid

   #119151

   Purpose

   Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1_sgRNA

   Plasmid

   #100554

   Purpose

   Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   AAVS1 sgRNA
   

   Promoter

   U6

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p105_gRNA_Sa_CD40

    Plasmid

    #213781

    Purpose

    Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pegRNA_LMNA_CR-2-5

    Plasmid

    #199275

    Purpose

    pegRNA used to correct LMNA (c.1824C > T) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LMNA 1824TtoC pegRNA
    

    Expression

    Mammalian

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCKO_Luciferase_sgRNA

    Plasmid

    #74190

    Purpose

    lentiviral vector expressing sgRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Luciferase sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. EC2_4_dCas9_VPR_sgRNA

    Plasmid

    #163709

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_sgRNA_MS2_neo

    Plasmid

    #118650

    Purpose

    To clone sgRNA for activation dCas9

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. M-NM-sgRNA

    Plasmid

    #48673

    Purpose

    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. px458_2A_GFP_sgRNA_RACK1

    Plasmid

    #127123

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA RACK1 (RACK1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6GRNA

    Plasmid

    #68370

    Purpose

    gRNA scaffold with hU6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. px458_2A_GFP_sgRNA_PELO

    Plasmid

    #127124

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA PELO (PELO Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available Since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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