169,450 results
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Plasmid#119040PurposeUAS-driven expresion of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionPromoterE1bAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF3180
Plasmid#144656PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-mScarlet3_N1
Plasmid#189767PurposeConstruct for labelling actin filaments in mammalian cells with lifeact-mScarlet3DepositorInsertLifeAct-mScarlet3
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFKB-TA-LUC-UBC-dTomato-W
Plasmid#49335PurposepHAGE NFKB-TA-LUC-UBC-dTomato-WDepositorInsertsNFkB consensus binding sequence
TA
Luciferase
UBC
dTomato
UseLentiviralAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF3274
Plasmid#144750PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8m
Plasmid#172388PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8m
UseNeurotropic virusAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseExpressionMammalianMutationNote that insert length is based on sum of the 2 …Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAB16 (pmyo-3::QuasAr)
Plasmid#130272PurposeExpresses the voltage sensor QuasAr in BWMs of C. elegans.DepositorInsertQuasAr
ExpressionWormAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pScaf-5544.4
Plasmid#111524PurposepScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)DepositorInsertpScaf-KH'
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB
Plasmid#171427PurposeExpression of HaloTag N58-(GGS)X6-FRB in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-FRB
ExpressionMammalianAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-mCh
Plasmid#129591PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag_HsIRE1a_deltaP29_D408_pBabePuro
Plasmid#58422Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids P29 to D408Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH127
Plasmid#162483PurposeSEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-PIP-FUCCI
Plasmid#138715PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianPromoterCMVAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2283
Plasmid#142008PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern(2x)
Plasmid#197929PurposeHomo-dimer expression vectorDepositorInsertmGreenLantern-mGreenLantern
ExpressionMammalianMutationnoneAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP Swe/Ind
Plasmid#30145DepositorInserthuman APP 695 Swedish/Indiana mutation (APP Human)
ExpressionMammalianMutationSwedish and Indiana; K595N, M596L and V642FAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
AiP5005 pAAV-Syn-DIO-TVA66T-dTom-SAD-B19G
Plasmid#176287PurposeCre-dependent AAV virusDepositorInsertTVA66T-dTomato-rabies Glycoprotein
TagsdTomatoExpressionMammalianAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GP736
Plasmid#164532PurposeExpress Smo-FLAG-UbDepositorAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_miRFP670
Plasmid#113726PurposeExpresses the fluorescent protein miRFP670 in a lentiviral backboneDepositorInsertmiRFP670
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mScarlet (AAV1)
Viral Prep#131001-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarletAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAXY0002
Plasmid#179835PurposeBinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorTypeEmpty backboneUseBinary vector for plant transformationExpressionPlantAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mNeongreen
Plasmid#169226PurposeTargeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mNeongreen
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3283
Plasmid#144759PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Strep-HA-VCF1 WT
Plasmid#223020PurposeExpression of Strep-HA-tagged VCF1 in mammalian cells.DepositorAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab7
Plasmid#214273PurposeMammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)DepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-globin_del5UTR_WT-xrRNA-4H
Plasmid#108366PurposeExpresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
ExpressionMammalianPromoterCMVAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (NZF1, aa 1-33)
Plasmid#65437PurposeExpresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coliDepositorInsertTRABID (ZRANB1 Human)
TagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-33 of TRABID, which …PromoterT7Available SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-SLC38A1_STOP
Plasmid#161261PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC38A1 (SLC38A1 Human)
ExpressionMammalianAvailable SinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-BACH1t_RFP
Plasmid#199214PurposeBACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.DepositorAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen2)
Plasmid#73498PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertsdCas9-KRAB
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPIC1.1
Plasmid#169897PurposeEmpty constitutive expression vector with piggyBac, IRES, and mClover3DepositorTypeEmpty backboneUseAvian expressionTags3x FLAG 2x StrepIIExpressionMammalianPromotermouse PGKAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hATG5 E122D
Plasmid#111804PurposeMammalian Expression of hATG5DepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_WDR77:1-342
Plasmid#211169PurposeMammalian expression of full-length human WDR77. C-terminal HiBiT tag.DepositorInsertWDR77:M1-E342 (WDR77 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
hChT pcDNA3
Plasmid#15766DepositorInserthemicholinium-3-sensitive choline transporter (SLC5A7 Human)
ExpressionMammalianAvailable SinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only