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UBQ10:sXVE:GFP1–10

Plasmid

#125663

Purpose

Binary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citron1

Plasmid

#134300

Purpose

Bacterial expression of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTK52_GFP-NuMA-C-3A

Plasmid

#46350

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115, and changed T…

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-H2Kb2

Plasmid

#17852

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

H-2Kb (H2-K1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mCherry

Plasmid

#191136

Purpose

expresses mCherry in mammalian cells

Depositor

Ken-ichirou Morohashi

Article

Inoue et al Commun Biol. 2022 Sep 15;5(1):974. doi:

Insert

mCherry

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CA

Plasmid

#69616

Purpose

To express gene strongly with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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AAV.CAG.GLPLight1

Plasmid

#187467

Purpose

Expresses GLPLight1 in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Promoter

CAG

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Hras

Plasmid

#180574

Purpose

ddRFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Hras

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSAD-F3-NPEST-iCRE-2A-mCherryPEST

Plasmid

#99608

Purpose

Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

iCRE
mCherry

Use

Unspecified

Tags

PEST

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6 BDNF

Plasmid

#39857

Depositor

Yves-Alain Barde

Article

Hofer et al EMBO J. 1990 Aug;9(8):2459-64.

Insert

BDNF (Bdnf Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUV15-pHGFP

Plasmid

#70045

Purpose

encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

Depositor

Sabine Ehrt

Article

Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

Insert

phGFP

Use

Mycobacterium tuberculosis expression vector

Promoter

mycobacterial promoter Psmyc

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1

Plasmid

#134301

Purpose

Bacterial expression of inverse-response citrate sensor with high binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Article

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pJB07

Plasmid

#190481

Purpose

Targeting plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Upstream & Downstream pyrE deletion region
gRNA

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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CMV::SypHy A4

Plasmid

#24478

Depositor

Leon Lagnado

Article

Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

Insert

SypHy (Syp Rat)

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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MSGV Hu Acceptor PGK-NGFR

Plasmid

#64270

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pECFP-DEVDR-Venus

Plasmid

#24537

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVDR-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
CTAG_mScarlet-I

Plasmid

#136118

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG-mScarletI(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MSGV Hu Acceptor

Plasmid

#64269

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

none

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP Tubulin K40R

Plasmid

#105303

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40R

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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V5-TurboID-Mfn1

Plasmid

#195155

Purpose

mammalian expression of TurboID tagged Mfn1

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

TurboID

Tags

Mfn1

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-HA.Tetherin

Plasmid

#41068

Depositor

Paul Spearman

Article

Ali et al PLoS One. 2010 Feb 2;5(2):e9005.

Insert

Tetherin (BST2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
FosB

Plasmid

#187908

Purpose

Expresses FosB in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

FosB (Fosb Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Rac1

Plasmid

#180575

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40A

Plasmid

#105304

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40A

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.hSyn.GLPLight1

Plasmid

#187466

Purpose

Expresses GLPLight1 in neurons

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Cdc42

Plasmid

#180578

Purpose

ddRFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CFP-CaaX

Plasmid

#155232

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Tags

CFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD119 (PCNA GFP)

Plasmid

#14822

Depositor

Robert Duronio

Article

Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Insert

PCNA-GFP (PCNA Fly)

Tags

GFP

Expression

Bacterial

Available Since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSJ111

Plasmid

#78707

Purpose

Modular vector for constitutive secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Expression

Bacterial

Promoter

Pveg

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Human Bcl-2/pcDNA3

Plasmid

#19279

Depositor

Philip Leder

Article

Fantin et al Cancer Res. 2004 Jan 1. 64(1):329-36.

Insert

human Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM sh2 DNMT1

Plasmid

#88885

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.2 DNMT1

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV (spleen focus forming virus)

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV (spleen focus forming virus)

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS167

Plasmid

#48881

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Inserts

araC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP1

Plasmid

#209988

Purpose

Entry vector to store Level 0 parts

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBV-S1-Luc

Plasmid

#71416

Purpose

To see S1 promoter activity of HBV for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S1 promoter driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV S1

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBV-S2-Luc

Plasmid

#71417

Purpose

To see HBV S2 promoter activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S2 promoter driving luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV S2

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b+H3

Plasmid

#157807

Purpose

Expression of cysteine-free human histone H3 (c111A)

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H3

Expression

Bacterial

Mutation

C111A

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3.1-NEFH

Plasmid

#26978

Depositor

David Sidransky

Article

Kim et al PLoS One. 2010 . 5(2):e9003.

Insert

NEFH (NEFH Human)

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 Plk4(Sak) wt (Nigg HR9)

Plasmid

#41165

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
iRFP-PAmCherry1-PH_PLCd1

Plasmid

#92167

Purpose

PI(4,5)P2 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PH_PLCd1

Tags

iRFP-PAmCherry1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAD-F3-NPEST-FLPo-2A-mCherryPEST

Plasmid

#99609

Purpose

Self-inactivating DG-Rabies (SiR) expressing FLPo and destabilised mCherry

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

FLPo
mCherry

Use

Unspecified

Tags

PEST

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO.puro_shHuR CDS

Plasmid

#110411

Purpose

TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

Depositor

Sandra Martha Gomes Dias

Insert

ELAVL1 (HuR)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pETduet+sfGFP-TetR

Plasmid

#157794

Purpose

Expression of sfGFP-TetR fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
JunB

Plasmid

#187903

Purpose

Expresses JunB in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

JunB (Junb Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK21

Plasmid

#37396

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Ran (RAN Human)

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

T24N

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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