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Showing: 4051 - 4100 of 159772 results

  9. pHAGE2-EF1aL-dsRed-W

    Plasmid
    #195863
    Purpose
    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter
    Depositor
    Darrell Kotton
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    dsRed
    Expression
    Mutation
    Promoter
    EF1aL
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195863-plasmid-map-sequence-id-383935

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  10. pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 (AAV9)

    Viral Prep
    #124650-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 (#124650). In addition to the viral particles, you will also receive purified pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 plasmid DNA. CamKIIa-driven expression of C1V1(t/t) fused to mScarlet fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Harvey
    Article
    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-
    Promoter
    CaMKII
    Tags
    mScarlet
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. pBMNb-cALFA

    Plasmid
    #159991
    Purpose
    For the protein expression with ALFA tag at C-terminus in mammalian cells
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Type
    Empty backbone
    Use
    Tags
    HRV3C protease cleavage site-ALFA tag-twin Strep …
    Expression
    Mammalian
    Mutation
    CMV promoter, WPRE
    Promoter
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159991-plasmid-map-sequence-id-314412

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  16. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    John Ngo
    Article
    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi
    Insert
    dCas9-NS3-NLS/VPR
    Use
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112244-plasmid-map-sequence-id-216918

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  20. TFORF1999

    Plasmid
    #143864
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TAL1 (TAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143864-plasmid-map-sequence-id-385795

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  21. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV9)

    Viral Prep
    #26969-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. C3AR1-DuET

    Plasmid
    #213191
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C3AR1 (C3AR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213191-plasmid-map-sequence-id-430756

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  26. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep
    #35509-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pD649-HAsp-OPCML-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156733
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    OPCML (OPCML Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156733-plasmid-map-sequence-id-434532

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  35. Human-Dysferlin-v1

    Plasmid
    #60217
    Purpose
    Human dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.
    Depositor
    Jain Foundation
    Insert
    Dysferlin (DYSF Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60217-plasmid-map-sequence-id-96331

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  40. TFORF1844

    Plasmid
    #144313
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RB1 (RB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144313-plasmid-map-sequence-id-404163

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  42. pminTolR4-R2

    Plasmid
    #67711
    Purpose
    mini Tol2 backbone with att4-attR2 cassette
    Depositor
    Nathan Lawson
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67711-plasmid-map-sequence-id-117439

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Showing: 4051 - 4100 of 159772 results