We narrowed to 23,545 results for: CHI

1. pGBW-m4046833

   Plasmid

   #145649

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp9

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. KA1158_pPBCAG-MerCreMer-IZ

   Plasmid

   #124184

   Purpose

   piggyBac-based expression of the tamoxifen-inducible Cre

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   MerCreMer-IRES-Zeo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2/V5 mmu-mir-122

   Plasmid

   #26325

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-122
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACS130

   Plasmid

   #204595

   Purpose

   Histone H3.1K9M-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.1 (H3C3 Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.1K9M-V5

   Expression

   Mammalian

   Mutation

   H3.1K9M

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PM18033-PLS-KP1_rpoA-U

   Plasmid

   #190975

   Purpose

   Test the U-to-C editing activity of KP1 on AtrpoA editing site, in human cells

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP1
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMpGWB100

   Plasmid

   #68062

   Purpose

   MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

   Plasmid

   #162804

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI_IRES_Blasticidin
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMB1-spect-PthrC-eGFP

   Plasmid

   #107408

   Purpose

   This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PthrC

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHA-ΔN331-Kin1B

   Plasmid

   #31603

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   ΔN331 kinesin-1B (Kif5b Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-331

   Promoter

   CMV

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCA-G-intron-G

    Plasmid

    #40027

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP exon 1
    GFP exon 2
    beta-globin intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insertion of a loxP site, nucleotides 1-274, and …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NKP64 GFP (1007)

    Plasmid

    #62020

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET-PLS-KP6_rpoA-C

    Plasmid

    #190966

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLPC-Flag-TRF2(delta)iDDR

    Plasmid

    #44577

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    TRF2ΔiDDR (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    iDDR domain (aa 407-431) has been deleted

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET22-HT/prsA

    Plasmid

    #73624

    Purpose

    Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

    Plasmid

    #124198

    Purpose

    Luciferase reporter driven by the Sox2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Sox2 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUM31dao

    Plasmid

    #87242

    Purpose

    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

    Depositor

    Anil Day

    Article

    Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

    Insert

    D-amino oxidase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    plastid rrn

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJL017

    Plasmid

    #53532

    Purpose

    This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpBA
    

    Use

    Tags

    6His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MPC020: CamKII CMV_NovArch_citrine

    Plasmid

    #153193

    Purpose

    Encodes Novarch

    Depositor

    Adam Cohen

    Article

    Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

    Insert

    NovArch
    

    Use

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2A-EKAREV-NLS

    Plasmid

    #173856

    Purpose

    Encoding EKAREV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKAREV-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPHAGE2-Myc-mTZAP

    Plasmid

    #87188

    Purpose

    lentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic stem cells

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (Zbtb48 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    March 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PM18033-PLS-KP6_rpoA-U

    Plasmid

    #190987

    Purpose

    Test the U-to-C editing activity of KP6 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046789

    Plasmid

    #145660

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046921

    Plasmid

    #145755

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pCMV Myc rat SAPAP3

    Plasmid

    #40217

    Depositor

    Yoshimi Takai, Yutaka Hata

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP3 (Dlgap3 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

26. NET39 pEGFP-C1 (1045)

    Plasmid

    #62024

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET39 (PPAPDC3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pGBW-m4046901

    Plasmid

    #145725

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. POM43-mCherry

    Plasmid

    #69857

    Purpose

    N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCherry minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KA1868_pGL4-UBCmin-luc2/Utf1-A'

    Plasmid

    #124194

    Purpose

    Luciferase reporter driven by the Utf1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Utf1 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p8126 MSCV-P C-FlagHA 16E7 R77S

    Plasmid

    #163305

    Purpose

    Expresses HPV16 E7 R77S

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    HPV16 E7 R77S
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    R77S

    Promoter

    MSCV LTR

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. aq-CFP

    Plasmid

    #36073

    Depositor

    Narasimhan Gautam

    Article

    Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

    Insert

    G protein subunit alpha q (Gnaq Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted between amino acid 124 and 125

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAGGS_2pabPAC

    Plasmid

    #158968

    Purpose

    2P-activatable bPAC for transient expression

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHA-ΔN332-Kin1C

    Plasmid

    #31608

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN332 kinesin-1C (Kif5c Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

36. p6993 MSCV-Neo C-HA 18E7

    Plasmid

    #163311

    Purpose

    Expresses HPV18 E7

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    HPV18 E7
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    MSCV LTR

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJB045 (ZapB-mEos2)

    Plasmid

    #72651

    Purpose

    Inducible expression of ZapB-mEos2 in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    ZapB-mEos2
    

    Use

    Tags

    mEos2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. KA1870_pGL4-UBCmin-luc2/Esrrb-B'

    Plasmid

    #124196

    Purpose

    Luciferase reporter driven by the Esrrb enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. KA1869_pGL4-UBCmin-luc2/Fgf4-A'

    Plasmid

    #124195

    Purpose

    Luciferase reporter driven by the Fgf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Fgf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGBW-m4046854

    Plasmid

    #145759

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pGBW-m4046832

    Plasmid

    #145691

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

    Plasmid

    #100979

    Purpose

    Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    c-Myc NLS-FKBP-Venus N-T2A-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGBW-m4046953

    Plasmid

    #145764

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. rat APOBEC1 E63A

    Plasmid

    #112861

    Purpose

    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 63 to Alanine

    Promoter

    CMV promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LC3-mEYFP

    Plasmid

    #182865

    Purpose

    Autophagy Marker

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    rat LC3 (Anxa3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCA-T-intron-G

    Plasmid

    #36887

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCA-G-intron-T

    Plasmid

    #36885

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGBW-m4046890

    Plasmid

    #145740

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge