We narrowed to 23,545 results for: CHI

1. pGBW-m4046841

   Plasmid

   #145578

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046883

   Plasmid

   #145693

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. KA1874_pGL4-UBCmin-luc2/Sox2_SRR1

   Plasmid

   #124197

   Purpose

   Luciferase reporter driven by the Sox2 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Sox2 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. KA2627_pGL4-UBCmin-luc2/Jam2-A'

   Plasmid

   #124200

   Purpose

   Luciferase reporter driven by the Jam2 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Jam2 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2

   Plasmid

   #124193

   Purpose

   Luciferase reporter driven by the Pou5f1 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Pou5f1 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3

   Plasmid

   #124192

   Purpose

   Luciferase reporter driven by the Pou5f1 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Pou5f1 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4

   Plasmid

   #124191

   Purpose

   Luciferase reporter driven by the Pou5f1 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Pou5f1 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KA1403_pGL4-UBCmin-luc2/Nanog-B'

   Plasmid

   #124190

   Purpose

   Luciferase reporter driven by the Nanog enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Nanog enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KA1402_pGL4-UBCmin-luc2/Nanog-A'

   Plasmid

   #124189

   Purpose

   Luciferase reporter driven by the Nanog enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Nanog enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PM18033-PLS-KP6_rpoA-C

    Plasmid

    #190986

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PM18033-PLS-KP2_rpoA-U

    Plasmid

    #190976

    Purpose

    Test the U-to-C editing activity of KP2 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PM18033-PLS-KP3_rpoA-U

    Plasmid

    #190980

    Purpose

    Test the U-to-C editing activity of KP3 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. POM42-mCitrine

    Plasmid

    #69854

    Purpose

    N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #128167

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-PLS-KP2_rpoA-U

    Plasmid

    #190956

    Purpose

    Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-PLS-KP6-A66S_rpoA-U

    Plasmid

    #190969

    Purpose

    Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-PLS-KP3_rpoA-U

    Plasmid

    #190960

    Purpose

    Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-PLS-KP6-E67A_rpoA-U

    Plasmid

    #190973

    Purpose

    Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA-ATG-intron-tdT3Myc

    Plasmid

    #36883

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tdTomato-3Myc
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    beta-globin intron containing a loxP site inserte…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NET39 mRFP-C1 (1219)

    Plasmid

    #62042

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET39 (PPAPDC3 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pPBpuro-LDR

    Plasmid

    #173857

    Purpose

    Encoding a part of the rapamycin-induced dimerization system.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    a Lyn11-targeted FRB (LDR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pACYC-Skp-hLYZ

    Plasmid

    #83924

    Purpose

    Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    lysozyme (LYZ Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046876

    Plasmid

    #145669

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046726

    Plasmid

    #145756

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pGBW-m4046945

    Plasmid

    #145668

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pET11a-DAAT-V33G/T242G

    Plasmid

    #159278

    Purpose

    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

    Depositor

    Roberto Chica

    Article

    Parmeggiani et al ACS Catalysis 9, 3482–3486.

    Insert

    Engineered d-alanine aminotransferase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Valine 33 and threonine 242 have been mutated to …

    Promoter

    T7

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGBW-m4046947

    Plasmid

    #145705

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pGBW-m4046804

    Plasmid

    #145595

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGBW-m4046766

    Plasmid

    #145612

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. acta 2

    Plasmid

    #78711

    Purpose

    zebrafish acta2 3-UTR–specific probe

    Depositor

    Sarah Childs

    Article

    Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

    Insert

    acta2 (acta2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains bp 1,243–1,591

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

31. p8141 MSCV-P C-FlagHA 18E7 D10K

    Plasmid

    #163306

    Purpose

    Expresses HPV18 E7 D10K

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    HPV18 E7 D10K
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    D10K

    Promoter

    MSCV LTR

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMB1-spect-PthrC3-eGFP

    Plasmid

    #107409

    Purpose

    This plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC3

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. POM43-mCitrine

    Plasmid

    #69855

    Purpose

    N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGBW-m4046926

    Plasmid

    #145663

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pcDNA3.1 myc Caronte (CT#612)

    Plasmid

    #13861

    Depositor

    Cliff Tabin

    Article

    Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

    Insert

    Caronte (CER1 Chicken)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFLAG Caronte (CT#304)

    Plasmid

    #13860

    Depositor

    Cliff Tabin

    Article

    Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

    Insert

    Caronte (CER1 Chicken)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAS1_4x7SKPylT_EF1_Ub-BsmBI

    Plasmid

    #162801

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBT241_(pCA-ATG-intron-tTA2)

    Plasmid

    #36875

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tTA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV_2pabPAC

    Plasmid

    #158969

    Purpose

    2P-activatable bPAC for AAV

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPAC
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGBW-m4046964

    Plasmid

    #145681

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pGBW-m4046950

    Plasmid

    #149258

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pET11a-TAX-L10-STEPtag

    Plasmid

    #159375

    Purpose

    Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    TAX-L10-STEPtag
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET11a-STEPtag-L10-TAX

    Plasmid

    #159374

    Purpose

    Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    STEPtag-L10-TAX
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pGBW-m4046971

    Plasmid

    #145664

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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45. pGBW-m4046755

    Plasmid

    #145573

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. 6his R.3-AR GUSpET28a+/pLysS

    Plasmid

    #61157

    Purpose

    N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

    Depositor

    Ichiro Matsumura, Andrew Ellington

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    GUSAR
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

    Promoter

    T7

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGBW-m4046905

    Plasmid

    #145762

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pGBW-m4046844

    Plasmid

    #145590

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. pGBW-m4046834

    Plasmid

    #149247

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pGBW-m4046908

    Plasmid

    #145603

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge