We narrowed to 58,897 results for: plasmid
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Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
TagsmEGFPExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-15F11-HA-Halo
Plasmid#129592PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
ExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-M (M SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET-28a_6H-MMLV_RT_D524N-6H
Plasmid#166945PurposeBacterial expression of M-MLV reverse transcriptaseDepositorInsertgag-pol
Tags6X HisExpressionBacterialMutationD524N, H8YPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP7f-WPRE
Plasmid#104488PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas serviceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-ChR2(H134R)-GFP
Plasmid#58880PurposeAAV expression of ChR2(H134R)-GFP under the Syn promoterDepositorHas serviceAAV Retrograde and AAV8InsertChR2(H134R)-GFP
UseAAVTagsGFPExpressionMammalianMutationH134RPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY109 (lenti-LbCpf1)
Plasmid#84740PurposeLenti virus delivery of LbCpf1 and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2APromoterEFSAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVPromoterChicken cardiac troponin TAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-Cre-GFP
Plasmid#68544PurposeAAV expression of Cre-GFP fusionDepositorInsertCre
UseAAV and Cre/LoxTagsEGFPExpressionMammalianPromoterCMVAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorHas serviceAAV5InsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseAAVTagsmCherryAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-ArchT-tdTomato
Plasmid#28305PurposeCre-dependent viral expression of ArchT-tdTomato for neuronal inhibitionDepositorHas serviceAAV5InsertArchT-tdTomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdTomatoExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human IFN-gamma promoter/enhancer
Plasmid#17598DepositorInsertIFN gamma promoter with 1st intron enhancer (IFNG Human)
TagsluciferaseExpressionMammalianMutation-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…Available sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
ExpressionMammalianAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas serviceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.GCaMP6f.WPRE.SV40
Plasmid#100836PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas serviceAAV1, AAV2, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by