We narrowed to 59,409 results for: plasmid
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Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas serviceAAV PHP.eBInsertGFP
UseAAVPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAcBac2.tR4-OMeYRS/GFP*
Plasmid#50831Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Tyr tRNA cassette
EGFP*
OMeYRS
two copy Tyr tRNA cassette
UseBaculoviralTagsHis, Myc-6xHis, and WPREExpressionInsectMutationY39TAG and able to charge various unnatural amino…PromoterCAG, CMV, and U6 and H1Available sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.27_ARE/NRF2-SPE
Plasmid#177775PurposeLuciferase reporter for ARE/NRF2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNRF2 (NFE2L2 Human)
UseLuciferaseAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.cTNT.iCre
Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorHas serviceAAV9Insertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…PromoterChicken cardiac troponin TAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-E (E SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas serviceAAV1InserttdTomato-T2A-SypEGFP
UseAAVPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCRISPR-EGFP
Plasmid#75159PurposeDerived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
TagsNoneExpressionBacterialMutationNonePromoterheat-shock inducibleAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pEF1a-DIO-FLPo-WPRE-hGHpA
Plasmid#87306PurposeAn AAV vector expresses FLPo in a Cre recombinase-dependent manner.DepositorHas serviceAAV1, AAV2, AAV5, AAV9, and AAV RetrogradeInsertCre-dependent mouse codon-optimized Flp recombinase.
UseAAVTagsN/AExpressionMammalianPromoterEF1aAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
TagsmEGFPExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.GFAP.iGluSnFr.WPRE.SV40
Plasmid#98930PurposeAAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVExpressionMammalianPromoterGFAPAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-M (M SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCMV-15F11-HA-Halo
Plasmid#129592PurposeEncodes anti-HA scFv (anti-HA frankenbody) fused with HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
ExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET-28a_6H-MMLV_RT_D524N-6H
Plasmid#166945PurposeBacterial expression of M-MLV reverse transcriptaseDepositorInsertgag-pol
Tags6X HisExpressionBacterialMutationD524N, H8YPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP7f-WPRE
Plasmid#104488PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas serviceAAV1, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7f
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-ChR2(H134R)-GFP
Plasmid#58880PurposeAAV expression of ChR2(H134R)-GFP under the Syn promoterDepositorHas serviceAAV8 and AAV RetrogradeInsertChR2(H134R)-GFP
UseAAVTagsGFPExpressionMammalianMutationH134RPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by