317,461 results

1. pDx_mScarlet3

   Plasmid

   #189754

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mRNA-CleanCap-mNeonGreen

   Plasmid

   #188248

   Purpose

   Template plasmid to transcribe mRNA encoding mNeonGreen

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mRNA-CleanCap-mNeonGreen

   Plasmid

   #188248

   Purpose

   Template plasmid to transcribe mRNA encoding mNeonGreen

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR_TRE3GS-->mCherry_PGK-->BFP

   Plasmid

   #162231

   Purpose

   Lentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   mCherry and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR_TRE3GS-->mCherry_PGK-->BFP

   Plasmid

   #162231

   Purpose

   Lentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   mCherry and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dCas9-miniTurbo

   Plasmid

   #219822

   Purpose

   EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-miniTurbo

   Plasmid

   #219822

   Purpose

   EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF525-EF1a-Hygro-P2A-mCherry-lenti

    Plasmid

    #115796

    Purpose

    Expresses mCherry in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available Since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCF525-EF1a-Hygro-P2A-mCherry-lenti

    Plasmid

    #115796

    Purpose

    Expresses mCherry in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available Since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMAL-7xHis-TEV-hexa

    Plasmid

    #222868

    Purpose

    Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

    Depositor

    Nikolaus Loening

    Article

    Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

    Insert

    TEV protease
    

    Use

    Tags

    7xHis and MBP

    Expression

    Bacterial

    Mutation

    C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-7xHis-TEV-hexa

    Plasmid

    #222868

    Purpose

    Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

    Depositor

    Nikolaus Loening

    Article

    Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

    Insert

    TEV protease
    

    Use

    Tags

    7xHis and MBP

    Expression

    Bacterial

    Mutation

    C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1_ADAR2DD_E488Q_Ago2

    Plasmid

    #205599

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    hAgo2 (AGO2 Human)
    

    Use

    Tags

    ADAR2DD_E488Q (hyperactive mutant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_ADAR2DD_E488Q_Ago2

    Plasmid

    #205599

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    hAgo2 (AGO2 Human)
    

    Use

    Tags

    ADAR2DD_E488Q (hyperactive mutant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

    Plasmid

    #60903

    Purpose

    Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Cas9 dead
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pR-spondin 1

    Plasmid

    #128844

    Purpose

    bacterial expression of pR-spondin 1

    Depositor

    Marc de la Roche

    Article

    Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

    Insert

    R-spondin 1 (RSPO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pR-spondin 1

    Plasmid

    #128844

    Purpose

    bacterial expression of pR-spondin 1

    Depositor

    Marc de la Roche

    Article

    Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

    Insert

    R-spondin 1 (RSPO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBad-CA

    Plasmid

    #105665

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBad-CA

    Plasmid

    #105665

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. modRNAc1-VP64dCas9VP64-2A-MPH

    Plasmid

    #216388

    Purpose

    this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    VP64dCas9VP64-2A-MPH
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. modRNAc1-VP64dCas9VP64-2A-MPH

    Plasmid

    #216388

    Purpose

    this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    VP64dCas9VP64-2A-MPH
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid

    #211942

    Purpose

    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

    Depositor

    Evgeny Kvon

    Article

    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSP68

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid

    #211942

    Purpose

    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

    Depositor

    Evgeny Kvon

    Article

    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSP68

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163055

    Purpose

    Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163055

    Purpose

    Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_ENVLPE+

    Plasmid

    #232427

    Purpose

    Expression plasmid to produce ENVLPE+ particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    ENVLPE+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xCMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Puro.Cre empty vector

    Plasmid

    #17408

    Purpose

    Lentiviral expression of Cre from the mouse PGK promoter

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available Since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Puro.Cre empty vector

    Plasmid

    #17408

    Purpose

    Lentiviral expression of Cre from the mouse PGK promoter

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available Since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 synthetic, Human)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge