161,697 results

1. pT7-EGFP-C1-HsTNRC6A_1-1208_M

   Plasmid

   #146943

   Purpose

   Mammalian Expression of HsTNRC6A_1-1208

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsTNRC6A_1-1208 (TNRC6A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF1473

   Plasmid

   #141826

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LIN54 (LIN54 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGal-Trans-Myc-eGFP-KDEL

   Plasmid

   #177729

   Purpose

   ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
   

   Use

   Tags

   KDEL, Myc, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pInducer20 CARD8-HA PAM E274R

   Plasmid

   #169984

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, E274R

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. R701-X72-527: His6-tev-Hs.CALM1(76-148)

   Plasmid

   #159695

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(76-148)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(76-148) (CALM1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHHM.X513-iNicSnFR3b-(V7)

   Plasmid

   #124881

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3b-(V7)
   

   Use

   Tags

   HA Tag, His Tag, and NA

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGP-AAV-syn-jGCaMP8m-WPRE

   Plasmid

   #162375

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-p300

   Plasmid

   #23252

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.hSyn.iGluSnFR3.v857.GPI (AAV1)

   Viral Prep

   #178331-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.GPI (#178331). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.GPI plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Promoter

   Tags

   None

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pBS-CMV-gagpol

    Plasmid

    #35614

    Depositor

    Patrick Salmon

    Insert

    MLV gag and pol genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JAK2 (V617F)-pcw107-V5

    Plasmid

    #64610

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    V617F

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV.rTH.PI.Cre.SV40 (AAV Retrograde)

    Viral Prep

    #107788-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    rTH

    Tags

    None

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Ef1a-fDIO-JRGECO1a (AAV1)

    Viral Prep

    #128317-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-JRGECO1a (#128317). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-JRGECO1a plasmid DNA. EF1a-driven, Flp-dependent expression of the biosensor jRGECO1a. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Promoter

    EF1a

    Tags

    None

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

15. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid

    #105550

    Purpose

    AAV expression of Cre from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB and AAV5

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Use

    Tags

    mito

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTNS2

    Plasmid

    #64968

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge