We narrowed to 6,030 results for: crispr cas9 expression plasmids
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Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable SinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42229PurposeThis plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
Plasmid#42333PurposeThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast (Lentiviral Prep)
Viral Prep#52962-LVPurposeReady-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.DepositorPromoterEFS-NSAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RSV-SpCas9 (AAV8)
Viral Prep#85450-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-RSV-SpCas9 (#85450). In addition to the viral particles, you will also receive purified pAAV-RSV-SpCas9 plasmid DNA. RSV-driven expression of SpCas9. These AAV preparations are suitable purity for injection into animals.DepositorPromoterpRSVAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9 (AAV8)
Viral Prep#104588-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EFS-SpCas9 (#104588). In addition to the viral particles, you will also receive purified pAAV-EFS-SpCas9 plasmid DNA. EFS-driven expression of Myc-tagged SpCas9. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEFSAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (AAV8)
Viral Prep#61592-AAV8PurposeReady-to-use AAV8 particles produced from pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (#61592). In addition to the viral particles, you will also receive purified pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA plasmid DNA. CMV-driven expression of SaCas9. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (AAV6)
Viral Prep#61592-AAV6PurposeReady-to-use AAV6 particles produced from pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (#61592). In addition to the viral particles, you will also receive purified pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA plasmid DNA. CMV-driven expression of SaCas9. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Jun lab tunable CRISPRi strain
Bacterial Strain#86400PurposeStrain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-termDepositorBacterial ResistanceSpectinomycin and hygromycinAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISV-CRISPR/Cas9PGmUbi
Plasmid#209189PurposeExpression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selectionDepositorInsertsCas9
DsRed(deltaB)
TagsNLSExpressionPlantMutationmissing BsaI site and plant-codon optimized with …PromoterCaMV 35S and GmUbi from Glycine max (L.) Merr. cv…Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN496_miniCRISPRcharm_dSaCas9
Plasmid#220837Purposemini CRISPRcharm expression in mammalian cellsDepositorInsertmini CRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEFSAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianPromoterLac promoter, SV40, and T7 promoterAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9/CD4-TK2
Plasmid#104397PurposeThe designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.DepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dual-CRISPR-Cas9-U6-H1 (pBP48)
Plasmid#218932PurposeThe backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.DepositorTypeEmpty backboneUseCRISPRTags2A-Puro and 3XHAExpressionMammalianAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only