420 results

1. RF13

   Bacterial Strain

   #62078

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. MG1655 ΔendA ΔrecA

   Bacterial Strain

   #37853

   Depositor

   Kristala Prather

   Article

   Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

   Bacterial Resistance

   None

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

3. BL21∆serB

   Bacterial Strain

   #34929

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Bacterial Resistance

   None

   Available since

   Feb. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Syn61Δ3(ev5) ΔrecA (ev1)

   Bacterial Strain

   #189857

   Purpose

   Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

   Depositor

   George Church

   Article

   Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

   Bacterial Resistance

   Streptomycin

   Species

   Synthetic

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

5. BJ5183 cells

   Bacterial Strain

   #16398

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Bacterial Resistance

   Streptomycin

   Species

   Escherichia coli

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. CJW4617

   Bacterial Strain

   #177118

   Purpose

   E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

   Depositor

   Christine Jacobs-Wagner

   Article

   Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

   Bacterial Resistance

   Kanamycin

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

7. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

8. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Bacterial Resistance

   None

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

9. EcNR2 Strain

   Bacterial Strain

   #26931

   Depositor

   George Church

   Article

   Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

   Bacterial Resistance

   Chloramphenicol and ampicillin

   Available since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

10. LC-E75

    Bacterial Strain

    #115925

    Purpose

    Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

11. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and tetracycline

    Available since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

12. BL21ΔBCF

    Bacterial Strain

    #102269

    Purpose

    lamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

13. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

14. B-95.ΔA

    Bacterial Strain

    #197933

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

15. JM110

    Bacterial Strain

    #49763

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    Streptomycin

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

17. rEcoli XpS

    Bacterial Strain

    #192872

    Purpose

    MG1655-C321 -- All 321 UAG codons changed to UAA

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

18. EcNR1 Strain

    Bacterial Strain

    #26930

    Depositor

    George Church

    Article

    Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

    Bacterial Resistance

    Ampicillin

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

19. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

20. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

21. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

22. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

23. DH10B-ALT

    Bacterial Strain

    #61151

    Purpose

    DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Bacterial Resistance

    None

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

24. BL21ΔCF

    Bacterial Strain

    #102265

    Purpose

    ompC, ompF double knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

25. JS200 strain

    Bacterial Strain

    #11794

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Bacterial Resistance

    None

    Available since

    June 20, 2007

    Availability

    Academic Institutions and Nonprofits only

26. JM107

    Bacterial Strain

    #49759

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

27. Corynebacterium glutamicum::RFP

    Bacterial Strain

    #79642

    Purpose

    Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Bacterial Resistance

    Gentamicin

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

28. ∆cosσε ∆HG

    Bacterial Strain

    #197132

    Purpose

    Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Bacterial Resistance

    Hygromycin

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

29. BL21ΔF

    Bacterial Strain

    #102259

    Purpose

    ompF deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

30. BL21ΔABC

    Bacterial Strain

    #102266

    Purpose

    ompA, lamB, ompC triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only