425 results

#26242

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Opher Gileadi

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Sept. 6, 2019

Academic Institutions and Nonprofits only

#174514

Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

Jason W Chin

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Nov. 18, 2021

Academic Institutions and Nonprofits only

#102270

ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

Jack Leo

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Nov. 10, 2017

Academic Institutions and Nonprofits only

#176581

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Chase Beisel, Jean-Loup Faulon

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Dec. 14, 2021

Academic Institutions and Nonprofits only

#51950

broad-host range natural conjugative plasmid

Farren Isaacs

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

May 1, 2014

Academic Institutions and Nonprofits only

#197113

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Christopher Voigt

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

June 30, 2023

Academic Institutions and Nonprofits only

#197132

Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

Baojun Wang

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

July 11, 2023

Academic Institutions and Nonprofits only

#108251

Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

Christopher Voigt

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

June 29, 2018

Academic Institutions and Nonprofits only

#230034

Fluorescently labelled (mCherry) E. coli

Tobias Bergmiller, Calin Guet

Bergmiller et al Science. 2017 Apr 21;356(6335):311-315.

Feb. 3, 2025

Academic Institutions and Nonprofits only

#102263

lamB, ompC double knockout strain for overproduction of outer membrane proteins

Jack Leo

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Nov. 10, 2017

Academic Institutions and Nonprofits only

#230031

Fluorescently labelled (YFP) E. coli

Tobias Bergmiller, Calin Guet

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Feb. 3, 2025

Academic Institutions and Nonprofits only

#115925

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

March 26, 2019

Academic Institutions and Nonprofits only

#197112

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Christopher Voigt

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

June 30, 2023

Academic Institutions and Nonprofits only

#230032

Fluorescently labelled (mCerulean) E. coli

Tobias Bergmiller, Calin Guet

Feb. 3, 2025

Academic Institutions and Nonprofits only

#156460

An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

Matthew D. Shoulders

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Oct. 19, 2020

Academic Institutions and Nonprofits only

#105063

host strain for phage assisted continuous evolution

David Liu

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

March 21, 2018

Academic Institutions and Nonprofits only

#192874

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Christian Chalut, Christophe Guilhot

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Sept. 7, 2023

Academic Institutions and Nonprofits only

#226851

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

David Nielsen

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Nov. 15, 2024

Academic Institutions and Nonprofits only

#87359

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

George Church

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

May 1, 2017

Academic Institutions and Nonprofits only

#176582

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Jean-Loup Faulon

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Dec. 14, 2021

Academic Institutions and Nonprofits only

#37853

Kristala Prather

Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

Nov. 6, 2012

Academic Institutions and Nonprofits only

#108254

Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

Christopher Voigt

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

June 26, 2018

Academic Institutions and Nonprofits only

#192872

MG1655-C321 -- All 321 UAG codons changed to UAA

Jesse Rinehart

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Dec. 14, 2022

Academic Institutions and Nonprofits only

#141407

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Antal Kiss

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Aug. 4, 2020

Academic Institutions and Nonprofits only

#182775

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

Michael Jewett

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

April 18, 2023

Academic Institutions and Nonprofits only

#218769

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Abhishek Chatterjee

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

June 21, 2024

Academic Institutions and Nonprofits only

#73581

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Farren Isaacs

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

March 4, 2016

Academic Institutions and Nonprofits only

#71852

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Todd Klaenhammer, Jan Kok

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Feb. 22, 2016

Academic Institutions and Nonprofits only

#189857

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

George Church

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Jan. 27, 2023

Academic Institutions and Nonprofits only

#65915

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

Keith Tyo

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

March 1, 2016

Academic Institutions and Nonprofits only