422 results

1. LC-E18

   Bacterial Strain

   #115924

   Purpose

   Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

2. BL21ΔF

   Bacterial Strain

   #102259

   Purpose

   ompF deletion strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   None

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

3. RF11

   Bacterial Strain

   #61961

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

4. sAJM.1505

   Bacterial Strain

   #108253

   Purpose

   Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Bacterial Resistance

   Chloramphenicol

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

5. BL21ΔABC

   Bacterial Strain

   #102266

   Purpose

   ompA, lamB, ompC triple knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   None

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

6. FW102 OL2-62

   Bacterial Strain

   #53735

   Purpose

   Bacterial-two hybrid reporter strain

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Bacterial Resistance

   Kanamycin and streptomycin

   Species

   Escherichia coli

   Available since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. CLM24 ∆lpxM

   Bacterial Strain

   #132780

   Purpose

   CLM24 strain with lpxM deletion

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

8. MG1655-OptoCre-knt-P*-R

   Bacterial Strain

   #188476

   Purpose

   Cre-inducible activation of knt for kanamycin resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Bacterial Resistance

   None

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

9. Jun lab tunable CRISPRi strain

   Bacterial Strain

   #86400

   Purpose

   Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

   Depositor

   Suckjoon Jun

   Article

   Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

   Bacterial Resistance

   Spectinomycin and hygromycin

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

10. RF12

    Bacterial Strain

    #62077

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. MM39

    Bacterial Strain

    #42894

    Depositor

    Bryan Berger

    Article

    Su et al J Biol Chem. 2012 Jul 22.

    Bacterial Resistance

    Streptomycin

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

12. USO hisB- pyrF- rpoZ-

    Bacterial Strain

    #18049

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Bacterial Resistance

    Tetracycline

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

13. bSLS.114

    Bacterial Strain

    #191530

    Purpose

    Contains a knockout gene in the E. coli strain BL21-AI

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

14. YM138

    Bacterial Strain

    #61953

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. TB205 △ilvA

    Bacterial Strain

    #230042

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for isoleucine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

16. TB204 △ilvA

    Bacterial Strain

    #230041

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for isoleucine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

17. TB205 △metA

    Bacterial Strain

    #230040

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for methionine

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Micali et al Proc Natl Acad Sci U S A. 2023 Nov 7;120

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

18. C321.∆A T7RNAP

    Bacterial Strain

    #182776

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

19. MutaT7

    Bacterial Strain

    #156460

    Purpose

    An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

20. US0hisB-pyrF-

    Bacterial Strain

    #12614

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Bacterial Resistance

    Tetracycline

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

21. C-5545 ∆cosδε

    Bacterial Strain

    #209018

    Purpose

    Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

    Depositor

    Alfonso Jaramillo

    Article

    Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

    Bacterial Resistance

    Hygromycin

    Species

    Escherichia coli

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

22. HE_150

    Bacterial Strain

    #201051

    Purpose

    DH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.

    Depositor

    Francis Nano

    Bacterial Resistance

    Streptomycin

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

23. DH10GFP

    Bacterial Strain

    #109392

    Purpose

    DH10B with a "capacity monitor" inserted in the lambda phage integration site.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Bacterial Resistance

    Kanamycin

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

24. RF6

    Bacterial Strain

    #62074

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

25. BL21ΔA

    Bacterial Strain

    #102256

    Purpose

    ompA deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

26. RF4

    Bacterial Strain

    #62072

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

27. TB205 △trpC

    Bacterial Strain

    #230038

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

28. TB204 △trpC

    Bacterial Strain

    #230037

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

29. TB205 △proC

    Bacterial Strain

    #230036

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for proline

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

30. TB204 △proC

    Bacterial Strain

    #230035

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for proline

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

31. Escherichia coli DH10B-MOB

    Bacterial Strain

    #229395

    Purpose

    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Bacterial Resistance

    60 ug/ml of dap

    Species

    Escherichia coli

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

32. MG1655-OptoCre-knt-P**-R

    Bacterial Strain

    #188477

    Purpose

    Cre-inducible activation of knt for kanamycin resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Bacterial Resistance

    None

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

33. KJBAC1 strain

    Bacterial Strain

    #13424

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Bacterial Resistance

    Tetracycline

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

34. BL21ΔACF

    Bacterial Strain

    #102268

    Purpose

    ompA, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

35. AV04

    Bacterial Strain

    #115926

    Purpose

    Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

36. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christophe Guilhot, Christian Chalut

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

37. C321

    Bacterial Strain

    #48999

    Purpose

    Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

38. E. coli MEV15

    Bacterial Strain

    #197112

    Purpose

    E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Bacterial Resistance

    None

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

39. HL 932

    Bacterial Strain

    #52940

    Purpose

    MG1655 + ΔlacI

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

40. MW005 (bacterial strain)

    Bacterial Strain

    #24545

    Depositor

    Colin Dolphin

    Article

    Westenberg et al BMC Biotechnol. 2010 Mar 29. 10(1):27.

    Bacterial Resistance

    Streptomycin

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only