419 results

1. Syn61

   Bacterial Strain

   #174513

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

   Depositor

   Jason W Chin

   Article

   Fredens et al Nature. 2019 May;569(7757):514-518. doi:

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

2. RF12

   Bacterial Strain

   #62077

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. bSLS.114

   Bacterial Strain

   #191530

   Purpose

   Contains a knockout gene in the E. coli strain BL21-AI

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

4. C321.∆A T7RNAP

   Bacterial Strain

   #182776

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

5. E. coli MEV20

   Bacterial Strain

   #197113

   Purpose

   E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

6. JM83

   Bacterial Strain

   #50348

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Messing et al Recombinant DNA Technical Bulletin, 1979

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. YM138

   Bacterial Strain

   #61953

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. USO hisB- pyrF- rpoZ-

   Bacterial Strain

   #18049

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

   Bacterial Resistance

   Tetracycline

   Available since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

9. KJ740(DE3)

   Bacterial Strain

   #179486

   Purpose

   KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Bacterial Resistance

   Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

   Species

   Escherichia coli

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

10. RF13

    Bacterial Strain

    #62078

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. JM101

    Bacterial Strain

    #50349

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Messing et al Recombinant DNA Technical Bulletin, 1979

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. TB202

    Bacterial Strain

    #230032

    Purpose

    Fluorescently labelled (mCerulean) E. coli

    Depositor

    Calin Guet, Tobias Bergmiller

    Bacterial Resistance

    None

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

13. TB205 △trpC

    Bacterial Strain

    #230038

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

14. TB204 △trpC

    Bacterial Strain

    #230037

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

15. TB205 △proC

    Bacterial Strain

    #230036

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for proline

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

16. TB204 △proC

    Bacterial Strain

    #230035

    Purpose

    Fluorescently labelled (GFP) E. coli, auxotrophic for proline

    Depositor

    Martin Ackermann, Olga Schubert

    Article

    Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

    Bacterial Resistance

    None

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

17. AV04

    Bacterial Strain

    #115926

    Purpose

    Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

18. MutaT7

    Bacterial Strain

    #156460

    Purpose

    An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

19. RF6

    Bacterial Strain

    #62074

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. BL21ΔA

    Bacterial Strain

    #102256

    Purpose

    ompA deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

21. US0hisB-pyrF-

    Bacterial Strain

    #12614

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Bacterial Resistance

    Tetracycline

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

22. DH10GFP

    Bacterial Strain

    #109392

    Purpose

    DH10B with a "capacity monitor" inserted in the lambda phage integration site.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Bacterial Resistance

    Kanamycin

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

23. MG1655GFP

    Bacterial Strain

    #109393

    Purpose

    MG1655 with a "capacity monitor" inserted in the lambda phage integration site.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Bacterial Resistance

    Kanamycin

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

24. HB101 endA::frt lambda pir

    Bacterial Strain

    #45472

    Depositor

    Michele Swanson

    Article

    Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

    Bacterial Resistance

    Streptomycin

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

25. HL 932

    Bacterial Strain

    #52940

    Purpose

    MG1655 + ΔlacI

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

26. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christian Chalut, Christophe Guilhot

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

27. LC-E02

    Bacterial Strain

    #78551

    Purpose

    E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Bacterial Resistance

    None

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

28. RF4

    Bacterial Strain

    #62072

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

    Bacterial Resistance

    None

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

29. JM106

    Bacterial Strain

    #49757

    Purpose

    E.coli K-12 derivative

    Depositor

    Joachim Messing

    Article

    Yanisch-Perron et al Gene. 1985;33(1):103-19.

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

30. BL21ΔACF

    Bacterial Strain

    #102268

    Purpose

    ompA, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

31. RF15

    Bacterial Strain

    #102799

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

32. S4197

    Bacterial Strain

    #200839

    Purpose

    wildtype strain MG1655 rph+, ilvG+, ΔlacZ

    Depositor

    Karin Schnetz

    Article

    Venkatesh et al J Bacteriol. 2010 Dec;192(24):6456-64. d

    Bacterial Resistance

    None

    Species

    Escherichia coli str. k-12 substr. mg1655

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

33. KJBAC1 strain

    Bacterial Strain

    #13424

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Bacterial Resistance

    Tetracycline

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

34. E. coli MG1655-Landing Pad-Clone 3

    Bacterial Strain

    #200007

    Purpose

    Landing pad (with pIP1433 seed promoter) in MG1655 clone 3

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Bacterial Resistance

    Apramycin

    Species

    Synthetic

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

35. C321

    Bacterial Strain

    #48999

    Purpose

    Recoded E. coli MG1655 strain with wt UAG termination function (RF1 is still present)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

36. E. coli MEV15

    Bacterial Strain

    #197112

    Purpose

    E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Bacterial Resistance

    None

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

37. MG1655 Z1 malE

    Bacterial Strain

    #65915

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

38. ADP1-ISx

    Bacterial Strain

    #216551

    Purpose

    Acinetobacter baylyi strain with transposons deleted from its genome

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Bacterial Resistance

    None

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

39. ML8

    Bacterial Strain

    #61909

    Purpose

    C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. argH gene deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

    Bacterial Resistance

    Kanamycin

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

40. SKA974

    Bacterial Strain

    #80403

    Purpose

    JT2-based E. coli host strain for optogenetic control of cell growth.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Bacterial Resistance

    Kanamycin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only