167,210 results

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pcDNA3.1-mRuby4(1-10)

Plasmid

#165143

Purpose

Expresses mRuby4(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

mRuby4(1-10)

Expression

Mammalian

Available Since

April 8, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mito-mScarlet

Plasmid

#141153

Purpose

Fluorescent labeling of the mitochondrial matrix in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

COX4 (COX4 Budding Yeast)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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JWW-2 human chimeric monoclonal antibody

Plasmid

#66749

Purpose

Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-2 Heavy Chain
JWW-2 Light Chain

Expression

Mammalian

Promoter

mEF1 and rEF1

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EC67844

Plasmid

#209514

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pV1093:SpRY

Plasmid

#172188

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Article

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pscAAV-GFP

Plasmid

#32396

Depositor

John T Gray

Article

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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mito-mStayGold_pTwistCMVBetaGlobWPRENeo

Plasmid

#221898

Purpose

Expresses mStayGold targeted to the mitochondria

Depositor

Janelia Integrative Imaging

Insert

cox8-mStayGold

Tags

Cox8

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-mArg1 promoter/enhancer -31/-3810

Plasmid

#34571

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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EF.CMV.RFP

Plasmid

#17619

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Expression

Mammalian

Promoter

EF1a/U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV Puro

Plasmid

#68469

Purpose

Retroviral plasmid with multiple cloning site and puromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Retroviral

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE2

Plasmid

#87085

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E2 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pALOD4 non-binding mutant

Plasmid

#111027

Purpose

Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A, G501A, T502A, T503A, L5…

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ153

Plasmid

#69304

Purpose

Gateway entry vector with pco-dCas9-3X(SRDX)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-3X(SRDX) (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

SRDX (X3) repressor fusion; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27

Plasmid

#163617

Purpose

mouse SNX27 cDNA N-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27 (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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BoxB in pcDNA3

Plasmid

#29727

Depositor

Howard Chang

Article

Wang et al Nature. 2011 Apr 7;472(7341):120-4. doi:

Insert

5BoxB

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pMax-E2F1

Plasmid

#16007

Depositor

Olimpia Meucci

Article

Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

Insert

E2F1 (E2F1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R (tumor-derived)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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