159,258 results

1. pRT029

   Plasmid

   #127969

   Purpose

   double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. v5 eVLP gag-pro-pol

   Plasmid

   #228466

   Purpose

   Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

   Depositor

   David Liu

   Article

   Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

   Insert

   MMLVgag-pro-pol(Q226P)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q226P

   Promoter

   CMV

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-OVA

   Plasmid

   #64599

   Purpose

   Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

   Depositor

   Martin Zenke, Sandra Diebold

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   Ovalbumin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_iPE-C_P2A_Csy4

   Plasmid

   #232428

   Purpose

   Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

   Insert

   iPE-C–P2A–Csy4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+HyPer7-NLS

   Plasmid

   #136468

   Purpose

   Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Triple nuclear localization signal of SV40 (simia…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZac2.1-GfaABC1D-Rpl22-HA

   Plasmid

   #111811

   Purpose

   Express Rpl22HA in astrocytes

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Ribosomal protein L22 (Rpl22 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-hM4D(Gi)-mCherry

   Plasmid

   #50475

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EFS-PEmax-P2A-Puro

   Plasmid

   #228890

   Purpose

   Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Inserts

   PEmax
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid

    #29655

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. RFP Dynamin2 Wild Type

    Plasmid

    #128152

    Purpose

    Expression of Dynamin 2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

    Insert

    Dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdTomato-PDHA1-N-10

    Plasmid

    #58124

    Purpose

    Localization: Mitochondria, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-pegRNA-puro backbone

    Plasmid

    #214085

    Purpose

    Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

    Plasmid

    #221225

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFX-Tom20-ｍCherry

    Plasmid

    #174188

    Purpose

    Express mCherry with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with mitchondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_cAMPFIRE-L

    Plasmid

    #182279

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)Flag-His-ATM wt

    Plasmid

    #31985

    Purpose

    Mammalian expression of human ATM with Flag and His tags

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM wild-type (ATM Human)
    

    Use

    Tags

    Flag and HIS

    Expression

    Mammalian

    Mutation

    3' UTR of 400 nucl.

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. p4xmts-mScarlet3_N1

    Plasmid

    #189774

    Purpose

    Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    4xmts-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPLICS LY-MT Short P2A

    Plasmid

    #213614

    Purpose

    Detect the short-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTW1169

    Plasmid

    #236583

    Purpose

    TRV1

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    TRV1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. v5 ABE-eVLP

    Plasmid

    #228467

    Purpose

    Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–ABE8e
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Expression

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGL4_CRE-CMVmin-luc2

    Plasmid

    #194384

    Purpose

    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    6xCRE-CMVmin-luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV minimal

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. evoBxb1

    Plasmid

    #222338

    Purpose

    Plasmid for expressing evolved Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-MMLVgag-3xNES-Cas9

    Plasmid

    #181752

    Purpose

    MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLV-Gag-3xNES-Cas9 (Spy)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pKL2299A

    Plasmid

    #222105

    Purpose

    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

    Depositor

    Kan Wang

    Article

    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

    Insert

    virA
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    virA promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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