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30,870 results

Showing: 181 - 200 of 30870 results

  3. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid
    #110824
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110824-plasmid-map-sequence-id-324008

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  5. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41732-plasmid-map-sequence-id-210671

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  7. GW1CMV-Perceval

    Plasmid
    #21737
    Purpose
    can be used to monitor the ATP:ADP ratio during live-cell imaging
    Depositor
    Gary Yellen
    Publication
    Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Perceval (fluorescent ATP:ADP sensor)
    Tags
    5'UTR
    Expression
    Mammalian
    Mutation
    improved Kozak sequence
    Available since
    Aug. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21737-plasmid-map-sequence-id-219888

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  8. mCherry-Lifeact-7

    Plasmid
    #54491
    Purpose
    Localization: Actin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Lifeact
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54491-plasmid-map-sequence-id-227430

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  11. pSQT1601

    Plasmid
    #53369
    Purpose
    human codon optimized Csy4 and FokI-dCas9 expression plasmid
    Depositor
    Keith Joung
    Publication
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53369-plasmid-map-sequence-id-127248

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  14. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61408-plasmid-map-sequence-id-333613

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  15. pEBFP2-Nuc

    Plasmid
    #14893
    Purpose
    Mammalian expression of EBFP2 blue fluorescent protein with NLS
    Depositor
    Robert Campbell
    Publication
    Ai et al Biochemistry. 2007 May 22;46(20):5904-10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEBFP2-Nuc
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14893-plasmid-map-sequence-id-122872

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  19. pSpCas9 (PX165)

    Plasmid
    #48137
    Purpose
    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48137-plasmid-map-sequence-id-130642

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Showing: 181 - 200 of 30870 results