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50 µg Transfection-Ready DNA Clear filter

27,007 results

Showing: 4001 - 4020 of 27007 results

  7. pCMV-PE7-P2A-BSD

    Plasmid
    #224122
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-BSD
    Tags
    P2A-BSD, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    224122-plasmid-map-sequence-id-442586

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  9. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  10. GPI-RpHLuorin2

    Plasmid
    #171721
    Purpose
    GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)
    Depositor
    Geert van den Bogaart
    Publication
    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ratiometric pHLuorin2 fused to GPI anchor
    Tags
    ratiometric pHluorin2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    171721-plasmid-map-sequence-id-355812

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  11. pcDNA3-cytoEKAR4

    Plasmid
    #174438
    Purpose
    Cytosol-targeted EKAR4.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytoEKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174438-plasmid-map-sequence-id-367724

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  12. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    168122-plasmid-map-sequence-id-332862

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  13. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161927
    Purpose
    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and Citrine repression reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    161927-plasmid-map-sequence-id-324349

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  14. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    157854-plasmid-map-sequence-id-307627

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  18. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Publication
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    118632-plasmid-map-sequence-id-230217

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  19. KRAB-dCas9

    Plasmid
    #112195
    Purpose
    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB (ZNF10 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-KRAB-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    112195-plasmid-map-sequence-id-213538

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Showing: 4001 - 4020 of 27007 results