Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 5941 - 5960 of 30881 results

  6. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

    Enlarge

  7. pcDNA3.1-Beta1

    Plasmid
    #140987
    Purpose
    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GBeta1 (GNB1 Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal human rhinovirus (HRV) 3C …
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140987-plasmid-map-sequence-id-301588

    Enlarge

  8. CMV-eGFP-Z1

    Plasmid
    #135049
    Purpose
    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Publication
    Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135049-plasmid-map-sequence-id-269408

    Enlarge

  11. pX330-sgR26

    Plasmid
    #127376
    Purpose
    Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.
    Depositor
    Lance Miller
    Publication
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ROSA26 (Gt(ROSA)26Sor Mouse)
    Use
    CRISPR and Mouse Targeting
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127376-plasmid-map-sequence-id-248005

    Enlarge

  13. pcDNA3.1(+)- e18

    Plasmid
    #131670
    Purpose
    Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.
    Depositor
    Alberto Ciccia
    Publication
    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
    Tags
    FLAG and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    131670-plasmid-map-sequence-id-257551

    Enlarge

  15. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

    Enlarge

  18. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid
    #101299
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.
    Depositor
    Alpha Yap
    Publication
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-Catenin
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-698 and 698-906 of alpha-C…
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101299-plasmid-map-sequence-id-246095

    Enlarge

  19. pcDNA3.1-CD40L-mgSrtA

    Plasmid
    #125795
    Purpose
    for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.
    Depositor
    P. Chen
    Publication
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD40L-mgSrtA
    Expression
    Mammalian
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125795-plasmid-map-sequence-id-245283

    Enlarge

Showing: 5941 - 5960 of 30881 results