31,685 results
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Plasmid#20857DepositorInsertBirA
Tags6xHisExpressionBacterialAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
ExpressionBacterialPromoterRhamnose promoterAvailable SinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
Tags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MZB211
Plasmid#228494Purpose10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)DepositorInsertpol
Tags10xHis-SUMO(Smt3)ExpressionBacterialMutationA408SPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJ4M/TDP-43
Plasmid#104480Purposebacterial expression of TDP-43DepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
Tags6X HisExpressionBacterialMutationE602DPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
ExpressionBacterialPromoterArabinose-inducible pBADAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialAvailable SinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHN924
Plasmid#178885PurposeExpression of GST-His8-HRV3CDepositorInserthuman rhinovirus 3C protease
TagsGST, His8ExpressionBacterialPromoterT7Available SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
Tags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348APromoteraraBADAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHT-ads
Plasmid#47382PurposeExpresses ads under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
Tags6xHisExpressionBacterialPromoterPgracAvailable SinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAT201
Plasmid#134878PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
TagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…ExpressionBacterialPromoterPvegAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHPSE-P6
Plasmid#185907PurposeEngineered Human Heparanase mutant for e.coli expressionDepositorTagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-ColH-HylB
Plasmid#248631Purposeexpresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coliDepositorInsertMyc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB
TagsHis and MycExpressionBacterialPromoteraraBADAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
ED_p_126
Plasmid#246073PurposeBacterial expression of ECOR31 2TMbeta residues 72-200 with N-terminal His-SUMO tag and co-expression of ECOR31 mCpolDepositorInsert2TMbeta
ExpressionBacterialAvailable SinceOct. 30, 2025AvailabilityAcademic Institutions and Nonprofits only