30,140 results

1. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Lars Axelsson

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a-BirA

   Plasmid

   #20857

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   BirA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSANG10-3F-BG4

   Plasmid

   #55756

   Purpose

   Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

   Depositor

   Shankar Balasubramanian

   Article

   Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

   Insert

   BG4
   

   Use

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. MP6

   Plasmid

   #69669

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-527

   Plasmid

   #11518

   Purpose

   Gateway destination vector for bacterial expression; His tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1142 (pSPIN, pBBR1 backbone)

   Plasmid

   #160730

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTagBFP2-pBAD

    Plasmid

    #54572

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, T7, and mTagBFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCRW1

    Plasmid

    #122462

    Purpose

    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells

    Depositor

    Richard C. Willson

    Article

    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10

    Insert

    NPM-ALK fusion protein (ALK, NPM1 Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1.Gαq-LgB97

    Plasmid

    #134360

    Purpose

    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαq-LgB97 (GNAQ Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-Skp-GroEL/ES

    Plasmid

    #83922

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Gro EL/ES
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTlpA_mCherry_YafQ/DinJ

    Plasmid

    #229148

    Purpose

    Toxin-Antitoxin (Dinj/YafQ)

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry, DinJ/YafQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtlpA (mCherry), DinJ/YafQ (Native)

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-anti-AlfaTag nanobody

    Plasmid

    #171819

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    tdTomato-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge