30,888 results

1. CRE recombinase

   Plasmid

   #62730

   Purpose

   Expresses Cre recombinase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21a-BirA

   Plasmid

   #20857

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   BirA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-MBP-TEV

   Plasmid

   #69929

   Purpose

   bacterial expression of MBP-TEV n-terminal fusions

   Depositor

   Zita Balklava, Thomas Wassmer

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   MBP and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mVenus-pBAD

   Plasmid

   #54845

   Purpose

   Localization: Protein Expression Vector , Excitation: 515, Emission: 528

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

   Type

   Empty backbone

   Tags

   6xHis and mVenus

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-28a(+)-FGF2-G3

   Plasmid

   #135521

   Purpose

   Engineered form of fibroblast growth factor 2 with improved thermostability

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   6x His tag and Agg Thrombin Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry-pBAD

   Plasmid

   #54630

   Purpose

   Localization: Protein Expression Vector , Excitation: 587, Emission: 610

   Depositor

   Michael Davidson, Nathan Shaner, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Tags

   6xHis and mCherry

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJ2072.2_CRISPRlator

   Plasmid

   #140674

   Purpose

   CRISPRlator

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   CRISPRlator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJLG038

    Plasmid

    #192981

    Purpose

    BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter

    Depositor

    Clay Fuqua

    Article

    Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

    Insert

    T7 polymerase
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TriEx-4 DHX36

    Plasmid

    #68368

    Purpose

    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

    Depositor

    James Vaughn

    Article

    Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    Histidine

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV ie enhancer/promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-EF1a-TetOn3G

    Plasmid

    #104543

    Purpose

    PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    Tet-On 3G
    

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S Synthetic, Human, SARS-COV-2)
    SARS-Cov-2 E protein (E Synthetic, Human, SARS-COV-2)
    SARS-Cov-2 M Protein (M Synthetic, Human, SARS-COV-2)
    

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available Since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pH2B_mCherry_IRES_puro2

    Plasmid

    #21045

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B (H2BC16P Human)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCyPet-His

    Plasmid

    #14030

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Insert

    CyPet
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJS169

    Plasmid

    #48882

    Purpose

    Negative feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

    Insert

    lacI with ssrA tag
    

    Use

    Synthetic Biology

    Tags

    ssrA (TSAANDENYALAA)

    Expression

    Bacterial

    Promoter

    Para/lac

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge