30,514 results

1. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFLP3

   Plasmid

   #64946

   Purpose

   Flp recombinase expression vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   Flp recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lambda promoter/temperature-sensitive Lambda cI r…

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGEX6P1-N-His

   Plasmid

   #119755

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   10xHis and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSANG10-3F-BG4

   Plasmid

   #55756

   Purpose

   Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

   Depositor

   Shankar Balasubramanian

   Article

   Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

   Insert

   BG4
   

   Use

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET23a-mUbc9

   Plasmid

   #53137

   Purpose

   E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Ubc9 (Ube2i Mouse)
   

   Use

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   protein sequence is identical to human

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEVS104

   Plasmid

   #207393

   Purpose

   Conjugal helper plasmid

   Depositor

   Edward Ruby, Eric Stabb, Jason Peters

   Article

   Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBbS5a-OptoCre-REDMAP

   Plasmid

   #228465

   Purpose

   Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

   Depositor

   Mary Dunlop

   Article

   Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

   Insert

   OptoCre-REDMAP
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacUV5

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNO286-3

   Plasmid

   #107746

   Purpose

   Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

   Depositor

   Jeffrey Tabor

   Article

   Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

   Inserts

   CcaS
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Deleted AA 223 and 512

   Promoter

   J23106 and J23108

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRAD1

    Plasmid

    #47697

    Purpose

    General cloning vector for use in E. coli or D. radiodurans

    Depositor

    Mary Lidstrom

    Article

    Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    repU

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJeM1

    Plasmid

    #135088

    Purpose

    rhamnose-inducible expression vector for replication in E. coli and P. putida

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTargetF

    Plasmid

    #62226

    Purpose

    Constitutive expression of sgRNA without donor editing template DNA

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pij23119

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKM208

    Plasmid

    #13077

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    lacI repressor (lacI E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcel and trc

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTNS3

    Plasmid

    #63127

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1 and Ptac

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge