30,535 results

1. HP17_KO7

   Plasmid

   #120346

   Purpose

   Helper plasmid for production of phagemid ssDNA, based on M13KO7

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Coding part of the M13KO7 helper phage genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   native phage promoters

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-SNAP-PI(3,4,5)P3 Probe

   Plasmid

   #211514

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BTK1-PH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9-CR4

   Plasmid

   #62655

   Purpose

   Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   Cas9 nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-SNAP-PI Probe

   Plasmid

   #211507

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   BcPI-PLC-ANH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-SNAP-PI(3,4)P2 Probe

   Plasmid

   #211511

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   TAPP1 cPHx3
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-SNAP-PI(4)P Probe

   Plasmid

   #211509

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   SidC (P4C)
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mVenus-pBAD

   Plasmid

   #54845

   Purpose

   Localization: Protein Expression Vector , Excitation: 515, Emission: 528

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mVenus

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbA6c-RFP

    Plasmid

    #35290

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Impact-T4gp43(exo-)

    Plasmid

    #228202

    Purpose

    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

    Depositor

    Stephen Benkovic

    Insert

    43
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Mutated aspartate 219 to alanine (D219A), elimina…

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHyo245

    Plasmid

    #173147

    Purpose

    Expresses pheS_A294G by dual T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD103

    Plasmid

    #119887

    Purpose

    Constitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

    Insert

    pConstitutive_mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7C-HSPA8

    Plasmid

    #177661

    Purpose

    Recombinant protein synthesis of human HSPA8

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHyo182

    Plasmid

    #173136

    Purpose

    Expresses pheS_A294G in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pheS_A294G
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroX-PTuner DD-linker-M.EcoGII

    Plasmid

    #122082

    Purpose

    Express M.EcoGII with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of M.EcoGII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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