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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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33,902 results

Showing: 2301 - 2320 of 33902 results

  2. pBad-HPII

    Plasmid
    #105839
    Purpose
    expresses E. coli HPII catalase with N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105839-plasmid-map-sequence-id-205523

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  4. XXBLM (XXBLMA-c075)

    Plasmid
    #98223
    Purpose
    Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag
    Depositor
    Nicola Burgess-Brown
    Insert
    XXBLMA (BLM Human)
    Tags
    His-6
    Expression
    Bacterial
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    98223-plasmid-map-sequence-id-193299

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  5. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Publication
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  8. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85502-plasmid-map-sequence-id-169860

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  11. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Publication
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-516189

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  13. MAPK1

    Plasmid
    #73248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK1 (MAPK1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73248-plasmid-map-sequence-id-137205

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  14. pNatA (pACYCduet-naa10-naa15)

    Plasmid
    #72928
    Purpose
    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker
    Depositor
    Dan Mulvihill
    Publication
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    naa10 (naa10 Synthetic, Fission Yeast)
    naa15 (naa15 Fission Yeast)
    Expression
    Bacterial
    Mutation
    Codon optomised for E.coli expression
    Promoter
    T7
    Available since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    72928-plasmid-map-sequence-id-194851

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  20. pDual-eGFP

    Plasmid
    #63215
    Purpose
    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 2301 - 2320 of 33902 results