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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMLRT

Plasmid

#78234

Purpose

Expresses Murine Leukemia Reverse Transcriptase (MLRT)

Depositor

Christopher Voigt

Article

Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

Insert

Murine leukemia reverse transcriptase

Expression

Bacterial

Promoter

J23102

Available Since

Aug. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pXint129

Plasmid

#178208

Purpose

Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

Depositor

Gregory Phillips

Article

Platt et al Plasmid. 2000 Jan . 43(1):12-23.

Insert

xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pSNAP-tag (T7) Vector

Plasmid

#101137

Purpose

Expression of protein of interest with SNAP-Tag in bacterial cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

SNAP-tag (SNAP26b)

Expression

Bacterial

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBad-PhoCl2c

Plasmid

#164035

Purpose

Improved PhoCl variant with enhanced dissociation efficiency

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl2c

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Article

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Sox10

Plasmid

#46536

Purpose

A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-6XHis-SUMO-SIRT2

Plasmid

#102622

Purpose

Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Tags

6XHis-SUMO

Expression

Bacterial

Mutation

Deleted amino acids 1-37 and 357-389

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINF

Plasmid

#26042

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His3C-lacZ

Tags

MAHHHHHHSSGLEVL

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag

Plasmid

#167274

Purpose

Overexpression of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cas9

Plasmid

#198275

Purpose

For loading SpCas9 into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cas9

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-parallel1-NLSH2BCas9

Plasmid

#112065

Purpose

Expression and purification of NLSH2BCas9 for fungal gene editing

Depositor

Jeff Coleman

Article

Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

Insert

NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger

Use

CRISPR

Tags

His tag

Expression

Bacterial

Mutation

Cas9 was codon optimized for Aspergillus niger

Promoter

promoter in vector

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pG1AK-sfGFP

Plasmid

#71739

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

sfGFP

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDS170-enNTS1

Plasmid

#199152

Purpose

JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

Depositor

Daniel Scott

Article

Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

Insert

enNTS1 (Ntsr1 Rat)

Tags

10xHis tag, Avi tag, MBP, and muGFP

Expression

Bacterial

Mutation

V208M, C273S, C391S and C393S (over NTS1-B5)

Promoter

Lac/T5

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLB038 - PHS1∆P

Plasmid

#136285

Purpose

entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

Depositor

Alexis Maizel

Article

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

PHS1∆P

Expression

Bacterial

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMTS-HA-GFP11

Plasmid

#177725

Purpose

Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

MTS-HA-GFP11

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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28A_APEX2_MSP1D1

Plasmid

#170923

Purpose

Express APEX2_MSP1D1 in Bl21

Depositor

Huan Bao

Article

Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

Insert

APEX2-MSP

Expression

Bacterial

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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