32,069 results
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Plasmid#153517PurposesgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertpnisA-sgRNA(EbpA_g1)-dCas9 scaffold
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS_flGAD
Plasmid#87031PurposeExpresses GAD (Glutamic Acid Decarboxylase) in BacteriaDepositorAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOCK5-1/1-IS1/2
Plasmid#91303PurposeProtein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2DepositorInsertDOCK5-1/1-IS1/2 (DOCK5 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-galK
Plasmid#193727PurposeContains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galKDepositorAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AFDN_PDZ_1
Plasmid#103938PurposeProtein expression and purification of TRITHORAX PDZ domainDepositorInsertAFDN (AFDN Human)
TagsGSTExpressionBacterialMutationcontains AA983-1110 of AFDNPromotertacAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM067
Plasmid#216820PurposeAspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold2
TagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-KOpstB
Plasmid#237501PurposeKnockout of pstBDepositorInsertPhosphate ABC transporter ATP binding subunit
ExpressionBacterialAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-KOpstC
Plasmid#237502PurposeKnockout of pstCDepositorInsertPhosphate ABC transporter ATP binding subunit pstC
ExpressionBacterialAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-URP114
Plasmid#237505PurposeUpregulation of mgdh with P114DepositorInsertmembrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
ExpressionBacterialMutationUpregulation of mgdh with P114Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKOS6b-URtufB
Plasmid#237506PurposeUpregulation of mgdh with tufBDepositorInsertmembrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
ExpressionBacterialMutationUpregulation of mgdh with tufBAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEB1-SCFP3A
Plasmid#103970PurposePlasmid encoding SCFP3ADepositorInsertSCFP3A
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD451-pA-APEX2
Plasmid#157863PurposeBacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labelingDepositorInsertpA-APEX2
ExpressionBacterialAvailable SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM069
Plasmid#216821PurposeAspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmStayGold2
TagsRIPGLIN and VinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1010 (pSL25)
Plasmid#191005PurposeExpresses blue chromoprotein on a broad host range RP4 origin plasmidDepositorInsertblue chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiHistoneNb
Plasmid#198293PurposeProduces PVCs retargeted with A9 anti-histone nanobodyDepositorInsertPVCpnf_pvc13-antiHistoneNb
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-human beta-catenin full length
Plasmid#132609Purposeexpresses human beta-catenin full length in E. coliDepositorInsertbeta-catenin
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
ExpressionBacterialMutationS111G/A137L/E263QAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher-mi3
Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
TagsC-tagExpressionBacterialPromoterT7Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-kimA-yfp
Plasmid#227638Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-624-919
Plasmid#104200PurposeExpresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion proteinDepositorAvailable SinceDec. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-POLR2A-CTD-mCherry
Plasmid#185011PurposeBacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion ProteinDepositorInsertPOLR2A (Polr2a Mouse)
ExpressionBacterialAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp10_SARS-CoV-2
Plasmid#167851PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRExpressionBacterial and YeastAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-Nedd4L
Plasmid#186806PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)
Plasmid#48322DepositorTypeEmpty backboneTagsHis6 and ThioredoxinExpressionBacterialAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIA1414
Plasmid#172402PurposeExpression of SARS-COV-2 Nsp9 in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-RNF126
Plasmid#138643PurposeBacterial expression of GST-RNF126 fusion proteinDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJ5
Plasmid#167134PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
IFI16-FL-(1-729)
Plasmid#35051DepositorAvailable SinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
hMSH2 Clone E3 (full-length)
Plasmid#16453DepositorInsertMSH2 (MSH2 Human)
ExpressionBacterialAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA_mTurquoise2
Plasmid#108237PurposeBroad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.DepositorInsertPsbA promoter - mTurquoise2
ExpressionBacterialAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-zif268 (omega)
Plasmid#18046DepositorInsertzif268 binding site
ExpressionBacterialMutationThe preferred binding site for zif268 is inserted…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-1
Plasmid#90325PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702DepositorInsertTetR
ExpressionBacterialAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS
Plasmid#244833PurposeBacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
R6K_tac_mCherry_ChInt
Plasmid#187382Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherryDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/human IL-8
Plasmid#17610DepositorInsertIL8 (CXCL8 Human)
ExpressionBacterialAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only