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Showing: 4641 - 4680 of 29646 results

  1. pCPP5910

    Plasmid
    #128717
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do
    Insert
    shcO-hopO1-1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128717-plasmid-map-sequence-id-252570

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  2. pCPP5058

    Plasmid
    #128710
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do
    Insert
    shcF-hopF2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128710-plasmid-map-sequence-id-252511

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  3. pOPINE-3C-eGFP

    Plasmid
    #41125
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41125-plasmid-map-sequence-id-54698

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  4. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Use
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  7. pSW149

    Plasmid
    #122248
    Purpose
    pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.
    Depositor
    Sarah Walker
    Article
    Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18
    Insert
    TIF4631 (TIF4631 Budding Yeast)
    Use
    Tags
    Intein-Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    122248-plasmid-map-sequence-id-245052

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  8. p5059

    Plasmid
    #198154
    Purpose
    bacterial expression of steC::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsteC steC::L16::HaloTag::HA
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198154-plasmid-map-sequence-id-386983

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  20. pMSR

    Plasmid
    #161983
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161983-plasmid-map-sequence-id-321920

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  22. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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  25. PylRS-AS

    Plasmid
    #137908
    Purpose
    For incorporation of fluorophenylalanine derivatives
    Depositor
    Wenshe Liu
    Article
    Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Containing N346A, C348S mutations
    Promoter
    pBAD
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    137908-plasmid-map-sequence-id-272329

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  26. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Use
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  32. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

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  33. pXW117As(RS-E11-RinA)2

    Plasmid
    #123146
    Purpose
    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123146-plasmid-map-sequence-id-243978

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  36. RBD#3L

    Plasmid
    #193020
    Purpose
    hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a
    Depositor
    Xue Gao
    Article
    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038
    Insert
    Cas13a
    Use
    Tags
    hnRNP A1 RRM2
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    193020-plasmid-map-sequence-id-380237

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Showing: 4641 - 4680 of 29646 results