30,719 results

1. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRBN_ΔHBD

   Plasmid

   #232779

   Purpose

   Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

   Depositor

   Ivan Dikic

   Article

   Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

   Insert

   Cereblon (CRBN Human)
   

   Tags

   MBP-His6-TEV

   Expression

   Bacterial

   Mutation

   CRBN (residues 47 to 193 and 249 to 436) with GNG…

   Promoter

   T7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBP-LbCas12a

   Plasmid

   #113431

   Purpose

   Expresses 10xHis-MBP fused to LbCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Has Service

   Cloning Grade DNA

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDx_mScarlet3

   Plasmid

   #189754

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-Skp-GroEL/ES

   Plasmid

   #83922

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Gro EL/ES
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-3z/601

   Plasmid

   #26656

   Depositor

   Jonathan Widom

   Article

   Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

   Insert

   
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD33.1

   Plasmid

   #36267

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA707

    Plasmid

    #186238

    Purpose

    KanR-BBR1 entry vector for HR donor

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a mScarlet

    Plasmid

    #178543

    Purpose

    The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mScarlet
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-PE2-His (IK1822)

    Plasmid

    #170103

    Purpose

    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

    Depositor

    Keith Joung

    Article

    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    

    Use

    CRISPR

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Bacteria codon-optimized nSpCas9 (H840A) & M-…

    Promoter

    T7

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUBC-popTag-QtE-mScarlet

    Plasmid

    #240101

    Purpose

    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

    Depositor

    Liam Holt

    Article

    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

    Insert

    Encapsulin
    

    Use

    Lentiviral

    Tags

    mScarlet and popZ

    Expression

    Bacterial and Mammalian

    Promoter

    UBC promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_TIA1

    Plasmid

    #106095

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Tags

    6xHIs-thrombin and T7

    Expression

    Bacterial

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE80l ACA

    Plasmid

    #196471

    Purpose

    Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    ACA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEP PolI

    Plasmid

    #11722

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I EP (polA E. coli)
    

    Expression

    Bacterial

    Mutation

    D424A, I709N, A759R. Error-prone version of PolI.

    Available Since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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