31,059 results
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Plasmid#29518DepositorInsertCUL1 (CUL1 Human)
TagsThrombin Cleavage SiteExpressionBacterialMutationN-terminal 415 aaAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
TRAV26-2
Plasmid#123399Purposeconstruction of TCRDepositorAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-4R
Plasmid#112090PurposeBacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.DepositorAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 NusA TEV LIC cloning vector (2N-T)
Plasmid#29709DepositorTypeEmpty backboneTagsHis6-NusA-TEVExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QNA
Plasmid#129305PurposeNegative control for ATP measurementDepositorInsertQUEEN-NA
ExpressionBacterialAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
P25-pZE-SUMO-PbTTA
Plasmid#204634PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-PbTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialPromoterPLtetO-1 promoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-His6_pET21a
Plasmid#199668Purposeexpresses LOV-Turbo in bacteriaDepositorInsertLOV-Turbo
TagsHis6ExpressionBacterialAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP33
Plasmid#199375PurposeEncodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPJ23116-MS2-rep
Plasmid#184842PurposeExpression of the rep protein of phage MS2DepositorInsertReplicase gene MS2 phage
UseSynthetic BiologyExpressionBacterialPromoterJ23116Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
His 3C MKK3 1-347
Plasmid#87878PurposeE. coli expression of MKK3 aa 1-347DepositorInsertMKK3 (MAP2K3 Human)
Tags3C protease site and 6xHisExpressionBacterialMutationaa 1-347PromoterT7Available SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PARP9-c020
Plasmid#173097PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEERM3 Km
Plasmid#64025PurposePlasmid for integration into neutral site of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPnrsBAvailable SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-WASP-GBD
Plasmid#30113DepositorTagsGSTExpressionBacterialMutationContains the Cdc42-interacting domain of WASP (aa…Available SinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
LIC1B_Caspase-LOVN7C-7
Plasmid#104629Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variationDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT23
Plasmid#89663Purposeexpression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT23
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST PTBP1 isoform 4
Plasmid#108594PurposeRecombinant GST tagged protein productionDepositorInsertPTBP1 isoform 4 (PTBP1 Human)
TagsGSTExpressionBacterialMutationisoform 4 (isoform a)PromotertacAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Dynamin 2 K44A
Plasmid#22301DepositorAvailable SinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRB035
Plasmid#181944PurposeaTc-inducible expression of mNeonGreenDepositorInsertmNeonGreen; tetR (AX04_RS23485 Synthetic)
ExpressionBacterialPromotermNG-PLtetO-1; tetR-J23106Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB1H1-Zif268
Plasmid#12612DepositorInsertZif268 in pB1H1 (Egr1 Mouse)
ExpressionBacterialAvailable SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1_enzymatic-domain
Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
Tags10xHis-MBP-TEVExpressionBacterialAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENV8(WT)-GFPuv
Plasmid#70127PurposeCobalamin riboswitch reporter vector for use in E. coliDepositorInsertwild type environmental cobalamin riboswitch sequence (env8)
UseSynthetic BiologyTagsNoneExpressionBacterialPromotertacAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT34
Plasmid#89669Purposeexpression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT34
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
rbcLS-pMAL-2px
Plasmid#41621DepositorInsertrbcL/rbcS
ExpressionBacterialPromoterPtacAvailable SinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
nuc-mCherry-pTRIP
Plasmid#163520PurposeUsed to construct lentivirus to express nucleus-targeted mCherry in mammalian cellsDepositorInsertnucleus targeted mCherry
UseLentiviralExpressionBacterial and MammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PpipB-GFPLVA
Plasmid#23344DepositorInsertGFP(LVA)
TagsGFP(LVA)ExpressionBacterialAvailable SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
AASDHPPT
Plasmid#39192PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHIP1-SH2
Plasmid#46492DepositorInsertSHIP1 (INPP5D Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-118PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015
Plasmid#202025PurposeExpresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coliDepositorInsertBacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
ExpressionBacterialMutationGvpB S9G-R31L-R85LAvailable SinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSG398
Plasmid#115604Purposesurface-displayed Nb3; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialMutationSee depositor comments belowAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
ExpressionBacterialPromoterPpro1; PlacIqAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (H6-tdTomato)
Plasmid#86928PurposepET LIC cloning vector for Biotin-His6-tev-yORF-tdTomatoDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and td…ExpressionBacterialPromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
Tags6x His tag and SUMO tagExpressionBacterialPromoterT7Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG030 (TerY-P)
Plasmid#157908PurposeExpresses the TerY-P defense systemDepositorInsertTerY-P triad (vWA + kinase + phosphatase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
ath5:GFP-CAAX
Plasmid#105958Purposetransgenesis, EGFP membraneDepositorInsertCAAX
TagsGFPExpressionBacterialAvailable SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-ASF1A
Plasmid#31591DepositorInsertASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
TagsHis and TEVExpressionBacterialAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_40
Plasmid#109239PurposePTac driving TALEsp2DepositorInsertTALEsp2
ExpressionBacterialPromoterPTacAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only