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pet26-MBP-ELIC
Plasmid#39239DepositorInsertEriwinia chrysanthemi - pentameric ligand gated ion channel
UseTagsHis-10 and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo1
Plasmid#25101DepositorInsertpro-SUMO1 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationChanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn E46K
Plasmid#105728PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein E46K (SNCA Human)
UseTagsExpressionBacterialMutationE46KPromoterT7Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT112TAG-SepT
Plasmid#34624DepositorInserttRNA^Sep
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU, aa 52-209)
Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFCyR2
Plasmid#167513PurposeBroad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertCymRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHARGE3
Plasmid#24658DepositorInsertTurbo
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAM552
Plasmid#154131PurposeExpresses wt rRNAs operon in bacterial cellsDepositorInsertwt bacterial rRNAs operon under control of pL promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ateam1.03-nD/nA/pRSETB
Plasmid#51960Purposebacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pHN767
Plasmid#178884PurposeExpression of bacterial PNGaseDepositorInsertPeptide:N-glycosidase F
UseTagsGST, His8ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitC
Plasmid#122596Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAH01
Plasmid#128162PurposeIncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
proISG15 (2-165)
Plasmid#110762PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX:PGRMC1
Plasmid#136062PurposeBacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.DepositorInsertProgesterone receptor membrane component 1 (PGRMC1 Human)
UseTagsGST-PGRMC1ExpressionBacterialMutationPromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL1M-pMtRC-MtLAP1-Adh-R1
Plasmid#170790PurposeA Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic StudiesDepositorInsertMtLAP1 (LOC11412013 Medicago truncatula)
UseTagsExpressionBacterial and PlantMutationPromoterMedtr4g059670(MtRC)Available sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb
Plasmid#198292PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobodyDepositorInsertPVCpnf_pvc13-antiMouseMHCIINb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110572PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertrhaSR-PrhaBAD-stRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPerimCh
Plasmid#227013PurposeExpresses periplasmic mCherryDepositorInsertPelB-mCherry, arabinose inducible cytosolic GFP
UseTagsExpressionBacterialMutationPromoterrpsM and pBADAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citron1
Plasmid#134300PurposeBacterial expression of direct-response citrate sensorDepositorInsertCitron1
UseTags6-His tagExpressionBacterialMutationPromoterpBADAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB235
Plasmid#164130PurposePhlF-RFP expression plasmidDepositorInsertPhlF-RFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
4R3H
Plasmid#64652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRFP-human APPL1
Plasmid#22202DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
UseTagsmRFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDD108
Plasmid#119884PurposeIntegration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP720
Plasmid#45460DepositorInsertiRFP720
UseTagsExpressionBacterialMutationT2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…PromoteraraBADAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
MP2
Plasmid#69628PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12089
Plasmid#222357PurposeTn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH5
Plasmid#199771PurposeExpresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)DepositorInsertcMSPdH5
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only