29,744 results

1. pLBH533_MBP-Cas14b2 expression

   Plasmid

   #112506

   Purpose

   Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21a.Tat(48-57)-I-SceI

   Plasmid

   #165041

   Purpose

   Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   I-SceI
   

   Use

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHis8-p100(1-899)

   Plasmid

   #44735

   Depositor

   Gourisankar Ghosh

   Insert

   p100 (NFKB2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AA 1-899

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

   Plasmid

   #114069

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS29: pDonor(PmcCAST)

   Plasmid

   #168162

   Purpose

   PmcCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST right end (RE)
   PmcCAST left end (LE)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBV514

   Plasmid

   #204602

   Purpose

   Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter

   Depositor

   Scott Moye-Rowley

   Insert

   Os Tir1 (LOC4335696 Oryza sativa)
   

   Use

   Tags

   Myc epitope tags

   Expression

   Bacterial

   Mutation

   Changed phenylalanine 74 into an alanine (F74A).

   Promoter

   ScADH1

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MacroH2A1.1-c002

   Plasmid

   #173087

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Q162-N369, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB1-PRD(800-868)

   Plasmid

   #141345

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1His6-TEV-ALIX(800-868)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7C-BAG2

   Plasmid

   #177654

   Purpose

   Recombinant protein synthesis of human BAG2

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   BAG2 (BAG2 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC-crRNA-Cm

    Plasmid

    #158711

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    eforCP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPROEX Hsp104 (1-575)

    Plasmid

    #1318

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (1-575) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa1-575 of Hsp104

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pThermoCas9i_ctrl

    Plasmid

    #100982

    Purpose

    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    changed aspartate 8 to alanine and histidine 582 …

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. DDX3XA

    Plasmid

    #42362

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DDX3XA (DDX3X Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pJJS008

    Plasmid

    #188331

    Purpose

    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::wrmScarlet::mIAA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Thomas Wassmer, Zita Balklava

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SQ171/pRibo-T

    Plasmid

    #69342

    Purpose

    SQ171 strain supported by pRibo-T plasmid

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    Ribo-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSA2

    Plasmid

    #52262

    Purpose

    Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKI1

    Plasmid

    #199118

    Purpose

    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    H6-SNAP-RapA
    

    Use

    Tags

    6xHis and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSL0915 (CRISPR with target 3)

    Plasmid

    #130643

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(target-3)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

    Plasmid

    #176745

    Purpose

    Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Glt1-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 6975 pPROEX-HTb-SIS1

    Plasmid

    #1233

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    sis1 (SIS1 Budding Yeast)
    

    Use

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

22. p11d-sctRPA

    Plasmid

    #102614

    Purpose

    Expression of S. cerevisiea RPA in E.coli

    Depositor

    Marc Wold

    Article

    Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

    Inserts

    RPA1 (RFA1 Budding Yeast)
    RPA2 (RFA2 Budding Yeast)
    RPA3 (RFA3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RFA2 has intron deleted

    Promoter

    T7

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQTEV-PSMD11

    Plasmid

    #31333

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. V621 pGEX-4T-LP - Acceptor

    Plasmid

    #11715

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 . 6():13.

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pET-His6-SUMO-MproC145A

    Plasmid

    #172653

    Purpose

    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

    Depositor

    Stephen Harrison

    Article

    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

    Insert

    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis Tag and SUMO

    Expression

    Bacterial

    Mutation

    Catalytic Cysteine 145 mutated to Alanine

    Promoter

    T7

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RP hk339-GFP

    Plasmid

    #24431

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin WT (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    monomeric kinesin

    Promoter

    Available since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 5TTW (EED)

    Plasmid

    #101254

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EED (EED Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCas1+2

    Plasmid

    #72676

    Purpose

    Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

    Depositor

    Udi Qimron

    Article

    Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

    Insert

    Cas1, Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b-CoilR-Cys

    Plasmid

    #181989

    Purpose

    Used to label proteins with the CoilE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    CoilR-Cys
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-GST-AGO1

    Plasmid

    #24316

    Depositor

    Carl Novina

    Article

    Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

    Insert

    human AGO1 (AGO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pET-Neq2X

    Plasmid

    #182365

    Purpose

    Expresses Neq2X DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSAN-QUE37C

    Plasmid

    #129312

    Purpose

    ATP measurement

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDD87

    Plasmid

    #119892

    Purpose

    The pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

    Insert

    pLPD03031_GFP+
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Use

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-5X1 Flag PA1

    Plasmid

    #15560

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    PA1 (PAGR1 Human)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid

    #122085

    Purpose

    Express M.EcoGII fused to CENPC with destabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of CENPC

    Expression

    Bacterial and Mammalian

    Mutation

    L341F, R930L (see comments below)

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p3990 pET ubcH8

    Plasmid

    #8650

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH8 (UBE2L6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

42. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

43. pTriEx-mPA-GFP-PA-Rac1

    Plasmid

    #22022

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mPA-GFP

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

44. pGGF_RT_FAST_Venus

    Plasmid

    #136980

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Insert

    seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

    Plasmid

    #171587

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS 6His

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    MKIEE-NLS-RfxCas13d-NLS-HA-6His
    

    Use

    Tags

    HA-6HIS and MKIEE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBT3011

    Plasmid

    #122539

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::cup-5 (cup-5 Nematode, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBbE8k-RFP

    Plasmid

    #35270

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. mt TEFM

    Plasmid

    #205058

    Purpose

    TEFM protein expression

    Depositor

    Smita Patel

    Article

    Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

    Insert

    TEFM (TEFM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCoofy2

    Plasmid

    #43981

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    Trx-His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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