32,776 results

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pE-SUMO CRYBA3

Plasmid

#80747

Purpose

Expression of human beta A3-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA3 (CRYBA1 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pWUR 1+2 tetO mut89

Plasmid

#80102

Purpose

Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
Cas1 (cas1 )
Cas2 (cas2 )

Expression

Bacterial

Mutation

Q24H, P202Q, G241D, E276D, L297Q

Available Since

Dec. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAWP78

Plasmid

#61263

Purpose

Small IncP-based broad host range replicating vector.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pQLinkH

Plasmid

#13667

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

His, TEV, and attB1

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

Plasmid

#160540

Purpose

Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Inserts

SARS-CoV-2 NSP7 (ORF1ab Synthetic)
SARS-CoV-2 NSP8 (ORF1ab Synthetic)
SARS-CoV-2 NSP12 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMMPc-Gm

Plasmid

#116921

Purpose

Gene constitutive expression from a Pc promoter

Depositor

Cuiqing Ma

Article

Xu et al Appl Environ Microbiol. 2013 Apr;79(8):2

Type

Empty backbone

Expression

Bacterial

Promoter

Pc promoter from Delftia acidovorans C17

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Expression

Bacterial

Promoter

COtetR-PIPL12

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBB528

Plasmid

#27390

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

lacIq

Expression

Bacterial

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23109

Plasmid

#113151

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

Plasmid

#140082

Purpose

Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

Depositor

Keith Joung

Insert

pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

Use

In vitro transcription (mrna synthesis)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRARE-MBP-DEST

Plasmid

#84650

Purpose

Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

Depositor

Neil Olszewski

Article

Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

tac

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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BRD4

Plasmid

#38943

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD4 (BRD4 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Article

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pNIC-HIS6-NUSA-TEV-GG

Plasmid

#215798

Purpose

Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pJK651

Plasmid

#71702

Purpose

Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

Depositor

T. J. Kappock

Insert

thioredoxin reductase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

ENO1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

Plasmid

#107581

Purpose

B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pdh promoter Bacillus megaterium DSM319

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAN4036

Plasmid

#74698

Purpose

Output plasmid: PPhlF-PAmtR-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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GST-tag ARIH2 binding E2 variant L3A2-1

Plasmid

#248581

Purpose

N-term GST tag E2 variant selected for activated ARIH2

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3A2-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag HOIP binding E2 variant L3HP-1

Plasmid

#248590

Purpose

N-term GST tag E2 variant selected for HOIP

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-L3HP-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-tag LUBAC binding E2 variant D3LU-2

Plasmid

#248591

Purpose

N-term GST tag E2 variant selected for LUBAC

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

pGEX-GST-D3LU-2

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-tag HOIL1 binding E2 variant L3HL-1

Plasmid

#248855

Purpose

N-term GST tag E2 variant selected for HOIL1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3HL-1

Tags

Avi and GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag LUBAC binding E2 variant L3LU-1

Plasmid

#248856

Purpose

N-term GST tag E2 variant selected for LUBAC

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3LU-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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32,776 results