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Bacterial Expression

29,747 results

Showing: 6751 - 6800 of 29747 results

  8. DT049A

    Plasmid
    #159410
    Purpose
    Constitutive murine mature BDNF expression, controlled under E.coli tet promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    tet promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    159410-plasmid-map-sequence-id-326573

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  14. pFEndoNA2

    Plasmid
    #174667
    Purpose
    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA2 fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174667-plasmid-map-sequence-id-343065

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  17. pAH25-SceI

    Plasmid
    #129389
    Purpose
    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Chloramphenicol acetyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    CAT promoter
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129389-plasmid-map-sequence-id-258595

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  20. pMARO

    Plasmid
    #178086
    Purpose
    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Insert
    TcR (tetA/tetR)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Tetracycline resistance cassette is flanked by Ec…
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178086-plasmid-map-sequence-id-349998

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  21. pGEX4T-3-6AOptParkin

    Plasmid
    #165107
    Purpose
    bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene
    Depositor
    Arthur Haas
    Insert
    Parkin isoform 1 (PRKN Human)
    Use
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165107-plasmid-map-sequence-id-326710

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  22. pGBW-m4046757

    Plasmid
    #145657
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145657-plasmid-map-sequence-id-298331

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  23. pSUMO-YHRC

    Plasmid
    #54336
    Purpose
    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.
    Depositor
    Claes Andréasson
    Article
    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d
    Type
    Empty backbone
    Use
    Tags
    6His-SUMO (yeast gene: SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7-LacO
    Available since
    Aug. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    54336-plasmid-map-sequence-id-84530

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  28. pBS_flGAD

    Plasmid
    #87031
    Purpose
    Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria
    Depositor
    Janice Blum
    Insert
    flGAD (GAD2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac-Z
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87031-plasmid-map-sequence-id-173237

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  30. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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  32. pET28-Mff(1-61)-PP-GST

    Plasmid
    #73042
    Purpose
    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Mff(1-61) (Mff Mouse)
    Use
    Tags
    GST-tag and PreScission protease site
    Expression
    Bacterial
    Mutation
    Truncation containing amino acids 1-61
    Promoter
    T7
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73042-plasmid-map-sequence-id-134011

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  33. R619-X67-527: His6-SARS-CoV-2 N

    Plasmid
    #170204
    Purpose
    E. coli expression of SARS-CoV-2 nucleocapsid protein
    Depositor
    Dominic Esposito
    Insert
    SARS-CoV-2 N (N SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170204-plasmid-map-sequence-id-334259

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  36. pBT3037

    Plasmid
    #122544
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Synthetic, Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122544-plasmid-map-sequence-id-238132

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  37. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

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  39. PP-G6PI

    Plasmid
    #28283
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-G6PI (PF14_0341 Plasmodium falciparum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    28283-plasmid-map-sequence-id-235428

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  41. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

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  47. pDB038

    Plasmid
    #99210
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*), luxAB; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at position 29 gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99210-plasmid-map-sequence-id-192356

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  48. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

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  49. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6751 - 6800 of 29747 results