Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,669 results

Showing: 6751 - 6800 of 32669 results

  3. LARS_pNIC-Bio3

    Plasmid
    #153052
    Purpose
    Expression construct for producing Avi tagged Leucyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    LARS (LARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Not full length protein
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153052-plasmid-map-sequence-id-304468

    Enlarge

  5. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Promoter
    T7-lac0
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  12. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

    Enlarge

  24. 4QQG

    Plasmid
    #61948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP:JMC001-B06:C41570 (KAT5 Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61272-plasmid-map-sequence-id-99585

    Enlarge

  35. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

    Enlarge

  36. pCut_GFP

    Plasmid
    #240154
    Purpose
    Expresses CRISPR guides
    Depositor
    Morten Norholm
    Article
    Gude et al bioRxiv 2025.06.19.660532
    Inserts
    constant cut sites CRISPR guide array
    "self-curing" CRISPR guide array
    GFP
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240154-plasmid-map-sequence-id-479867

    Enlarge

  42. pET28a(+)/FbGH17B

    Plasmid
    #100912
    Purpose
    Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli
    Depositor
    Jan-Hendrik Hehemann
    Insert
    FbGH17Bcat
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    not full length
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    100912-plasmid-map-sequence-id-193695

    Enlarge

Showing: 6751 - 6800 of 32669 results