32,669 results
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Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
ExpressionBacterialMutationSynonymous point mutation in the wobble position…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-CitA-Tq-LR
Plasmid#241830PurposeBacterial expression vector for CitA-Tq-LR (low-range)DepositorInsertCitA-Tq-LR
Tags7xHisExpressionBacterialAvailable SinceOct. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-CitA-Tq-HR
Plasmid#241831PurposeBacterial expression vector for CitA-Tq-HR (high-range)DepositorInsertCitA-Tq-HR
Tags7xHisExpressionBacterialAvailable SinceOct. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-SENP1
Plasmid#196354Purposeprotein expression of the His-tagged catalytic domainDepositorInsertSENP1 catalytic domain
TagsHis6-TEVExpressionBacterialMutationcontains only E419-L644PromoterT7Available SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
JMJD3
Plasmid#39069PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2L6
Plasmid#15797DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
PANK3
Plasmid#32872PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPANK3 (PANK3 Human)
TagsHis tag (with Thrombin cleavage)ExpressionBacterialMutationamino acids 10-370 onlyAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Nano-lantern/pRSETB
Plasmid#51969Purposebacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imagingDepositorInsertNano-lantern
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available SinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1
Plasmid#68300PurposeN-terminal MBP tagged human MEK1 gene for bacterial expressionDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB064d
Plasmid#79197PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlac62; Ppro1Available SinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTIII.EFGP.IGR3.6
Plasmid#40866DepositorAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT86
Plasmid#108965PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tagDepositorTypeEmpty backboneTagsOmpA 1-27, 6HisExpressionBacterialPromoterT7Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-4HA
Plasmid#17375DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X2-RalBP1
Plasmid#37245DepositorAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)
Plasmid#15384DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS123…Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJB042 (FtsZ-mEos2)
Plasmid#49764PurposeInducible expression of FtsZ-mEos2 in bacteriaDepositorInsertFtsZ-mEos2
TagsmEos2ExpressionBacterialPromoterT5-lacAvailable SinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
R6K_tac_dGFPmut3_ChInt
Plasmid#187388Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
TagsAAVExpressionBacterialMutationC-terminal AAV tailAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pAOX1
Plasmid#98514PurposeBB1 with insert: promoter PAOX1DepositorInsertAOX1 promoter
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL8B
Plasmid#67383PurposeExpression of native hArl8b in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATLIC
Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
ExpressionBacterialPromoteraraBAD promoterAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pWM_12x601_193 NRL + TetO
Plasmid#157812PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_tetO
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK204
Plasmid#13534DepositorInsertFLIPW-CTYT
TagsSee mapExpressionBacterialAvailable SinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
Tags6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone45)
Plasmid#188578PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
ExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK002
Plasmid#65109PurposeEncodes ConLS as a Type 1 part to be used in the Dueber YTK systemDepositorInsertConLS
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMyBADC
Plasmid#84688PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseBacterial expression vectorTagsHis6ExpressionBacterialPromoterArabinoseAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUX19
Plasmid#36328DepositorTypeEmpty backboneUseSuicide vector for r. rubrumExpressionBacterialPromoterreduce strength lacZAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
ExpressionBacterial, Mammalian, and Y…Available SinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease
Plasmid#228637PurposeBacterial expression of the Coxsackievirus 16 3C proteaseDepositorInsertCoxsackievirus 16 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJKR-H-mphR
Plasmid#62559PurposeMacrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAraGFPCDF
Plasmid#47516PurposeContains PBAD::GFP reporter, used for heterodimer competition assayDepositorInsertGFP
ExpressionBacterialAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
lacI-PT7-gusA-pBAV1k
Plasmid#30504DepositorAvailable SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
ExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos16)GFP
Plasmid#89249PurposeExpresses GFP with +16 theoretical charge in E.ColiDepositorInsertHis6-(pos16)-GFP
TagsHis6ExpressionBacterialPromoterT7Available SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM11-ACS
Plasmid#108943Purposeheterologous expression of ACS of E. coli in E. coliDepositorInsertAcetyl-CoA synthetase (ACS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSA1
Plasmid#52261PurposeBacterial expression of human 6His-tagged SUMO1 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS10-ZBASIC-TEV-GG
Plasmid#215797PurposeCloning of target gene with N-terminal His10 Zbasic tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-NoTag-GG
Plasmid#215810PurposeCloning of target gene with no tagsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCDC124-Halo
Plasmid#175328PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_2
Plasmid#103943PurposeProtein expression and purification of PDZK1 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only