Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,059 results

Showing: 6851 - 6900 of 31059 results

  2. pQE81XN-msYFP

    Plasmid
    #218960
    Purpose
    Expresses Hisx6-Tagged msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP
    Tags
    6X-His
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218960-plasmid-map-sequence-id-446223

    Enlarge

  13. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

    Enlarge

  14. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Tags
    His and mRuby3
    Expression
    Bacterial
    Promoter
    BBa_J23118 - Strong Constitutive
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

    Enlarge

  15. pEXP5-NT/pLux eGFP

    Plasmid
    #193624
    Purpose
    Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    eGFP
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLux
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193624-plasmid-map-sequence-id-381997

    Enlarge

  16. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

    Enlarge

  19. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

    Enlarge

  24. Otx1/pBS

    Plasmid
    #16332
    Depositor
    Peter Klein
    Insert
    Otx1 (otx1 Frog)
    Expression
    Bacterial
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  26. pKB231

    Plasmid
    #170018
    Purpose
    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Cecropin P1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170018-plasmid-map-sequence-id-336783

    Enlarge

  27. SUV420H2

    Plasmid
    #32864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV420H2 (KMT5C Human)
    Tags
    His tag (with TEV protease)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. pET-Sac-Abeta(MC1-40)

    Plasmid
    #127152
    Purpose
    Expresses N-terminal cysteine Abeta(1-40)
    Depositor
    James Nowick
    Insert
    Abeta(MC1-40) (APP Human)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127152-plasmid-map-sequence-id-247836

    Enlarge

  31. pBS-AMVpl-γWT-nAchR

    Plasmid
    #97187
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97187-plasmid-map-sequence-id-183032

    Enlarge

  32. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

    Enlarge

  42. pCPP5912

    Plasmid
    #128703
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcE-avrE1
    Expression
    Bacterial
    Mutation
    Three synonymous mutations Ala 362 (GCT-GCC), Ala…
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128703-plasmid-map-sequence-id-252488

    Enlarge

  45. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

    Enlarge

  46. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

    Enlarge

  49. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

    Enlarge

Showing: 6851 - 6900 of 31059 results