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Plasmid#62329PurposeE. coli pACYC based expression vector with N-terminal TEV cleavable GST-tagDepositorTypeEmpty backboneUseTagsGlutathione S-transferase (GST) tag and TEV proteā¦ExpressionBacterialMutationPromoterT7-lac0Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only
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pMG046 pETARA-CK1α-His
Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-LSSmCherry1(-13)
Plasmid#219415PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertLSSmCherry1(-13)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199EPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTB3-Myc tag
Plasmid#210205PurposeType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.DepositorInsertMyc-tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB2a
Plasmid#190120PurposeLevel 2 MoClo plasmid for A. baumanniiDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-moxMaple3
Plasmid#120871PurposeBacterial expression of moxMaple3DepositorInsertmoxMaple3
UseTagsHisExpressionBacterialMutationfrom mMaple3, moxMaple3 was engineered by replaciā¦PromoterAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
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DT044A
Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterT7/LacO promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX TIF1gamma
Plasmid#15734DepositorInsertTIF1 gamma (TRIM33 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD103
Plasmid#119887PurposeConstitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-MBP-NLS-RfxCas13d-NLS-HA
Plasmid#171586PurposeBacterial expression of NLS-RfxCas13d-NLS-HADepositorInsertHis-MBP-NLS-RfxCas13d-NLS-HA
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBL132
Plasmid#104333PurposeExpresses taz from a LacI regulated promoter under inducible IPTG control.DepositorInsertTaz
UseTagsExpressionBacterialMutationPromoterPtacIAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV537
Plasmid#177704PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida luā¦Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTG2
Plasmid#149475PurposeBroad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertTetR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTetAAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-eBFP2
Plasmid#102640PurposeIn vitro expression of eBFP2 from the T7 promoterDepositorInserteBFP2
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II
Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &ā¦PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-D4
Plasmid#37473DepositorInsertD4 second generation cameleon (calcium sensor)
UseTagsExpressionBacterialMutationPromoterAraAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDM098
Plasmid#216818PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold
UseTagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3ExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUP1
Plasmid#223516Purposeinactive cogfp under control of Ptet, cogfp under control of PtacDepositorArticleInsertsCoGFP
CoGFP inactivated
UseTagsHis tagExpressionBacterialMutationanti-dimerization mutations S129G, 22 C154S, D156ā¦PromoterPtac, Ptac and Ptet, and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7AW)RS
Plasmid#207516PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-bapaUGAc
Plasmid#201673Purposeboronic acid- and peptide-assisted UDP-GlcNAc sensorDepositorInsertbapaUGAc
UseTagsHisx6ExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)
Plasmid#197660PurposeExpresses GFP under the control of J23100 and BCD2DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RAB5A_Ras
Plasmid#109935PurposeProtein expression and purification of RAB5A_RasDepositorInsertRAB5A_Ras
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJF093
Plasmid#113323PurposeSp.pCas9-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUS252y
Plasmid#191834PurposeExpresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.DepositorInsertfuYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKL002
Plasmid#98921PurposepET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.DepositorInsertsPROPS
GCaMP6f
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -