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Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTE1062
Plasmid#172356PurposeDetection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.DepositorInsertsUseSynthetic Biology; Integrative into streptomyces …TagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X1 Flag RbBP5
Plasmid#15551DepositorInsertRbBP5 (RBBP5 Human)
UseTagsFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA5-lenti
Plasmid#115664PurposeExpresses AcrVA5 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA5
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
R725-X11-566: His6-MBP-tev-Hs.LGALS3
Plasmid#159699PurposeE. coli protein expression of His6-MBP-tev-Hs.LGALS3DepositorInsertHis6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNAmreB
Plasmid#149654Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-TEV-HsRCK_296-472_E
Plasmid#146209PurposeBacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DVLP1_DEP
Plasmid#109806PurposeProtein expression and purification of DVLP1_DEPDepositorInsertDVLP1_DEP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri-18a
Plasmid#61326PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-4
Plasmid#13001DepositorInsertTSP-4 (THBS4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Smurf2 wt
Plasmid#13504DepositorInsertSMURF2 (SMURF2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
28 pCOLI_S_STREP
Plasmid#160262PurposeN-term 2xStrepII 3C cleavable Spec resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDM067
Plasmid#216820PurposeAspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmStayGold2
UseTagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZE31-DsRed-ms2x2
Plasmid#26248DepositorInsertms2 binding sites (x2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pAC1749
Plasmid#127983PurposeAMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSA-C
Plasmid#17329DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
Rec-PhoA/CsgA
Plasmid#170787PurposeRecombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinaseDepositorUseSynthetic BiologyTagsHHHHHHExpressionBacterialMutationPromoterInverted proD with Bxb1recognition sites and Inve…Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQFT
Plasmid#203674PurposepQF derivative with promoter PQ5 exchanged for promoter PQJDepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
UseTagsExpressionBacterial and YeastMutationPromoterpombe TEFAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
UseTagsFLAG purification tag and eGFPExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-MMLV-RT
Plasmid#207457Purposein vitro transcription of mRNADepositorInsertMCP-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.mCherry
Plasmid#112520PurposeProB driven mCherry expression with synTTDepositorInsertProB mCherry TT
UseTagsExpressionBacterialMutationPromoterProBAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
Plasmid#172501PurposeEncodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacersDepositorInsertDisCas7-11 full locus with Golden gate acceptor site for spacer
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM34
Plasmid#89156PurposeRecombinant expression of PTBP1-RRM34 in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
UseTagshexa HisExpressionBacterialMutationcontains RNA Recognition Motif 3+4PromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE30-rSyntenin1
Plasmid#89428Purposebacterial expression of rat Syntenin1DepositorInsertSDCBP
UseTagsHisExpressionBacterialMutationPromoterT5Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMevB
Plasmid#17819DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJMP2844
Plasmid#160675PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-157
Plasmid#118539PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMO746
Plasmid#117480Purposeupp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanRDepositorInserturacil phosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2H
Plasmid#15752DepositorInsertUbiquitin conjugating enzyme UbE2H (UBE2H Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
MBP-CHC(1-1074)-His6
Plasmid#100748PurposeBacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.DepositorInsertCLTC (CLTC Human)
UseTagsHis and MBPExpressionBacterialMutationResidues 1-1074PromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVL.Active/Repressed TLR (Sce)
Plasmid#45575PurposeCodes for the Active/Repressed TLR with the target site for I-Sce I nucleaseDepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only