32,156 results
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Plasmid#15791DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only
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pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)
Plasmid#114073PurposeT7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548R and N282AAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130A wgMDH
Plasmid#204266PurposeBacterial expression of R130A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:fflucRT
Plasmid#88901PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + Red-shifted firefly luciferase
ExpressionBacterialAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher
Plasmid#216284PurposeExpresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked bindersDepositorInsertDoubleCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM2 hTSP-1
Plasmid#12993DepositorInsertTSP-1 (THBS1 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pDSG375
Plasmid#115603Purposesurface-displayed Nb2; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002
Plasmid#111248PurposeBroad host-range bacterial vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYUBDuet
Plasmid#37278DepositorTypeEmpty backboneUseMycobacteria shuttle vectorTagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS
Plasmid#244833PurposeBacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
TagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1153
Plasmid#222930PurposeShuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable markerDepositorInsertCEN 4
ExpressionBacterialAvailable SinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
6xHis-MBP-huMb3Cas12a
Plasmid#174677PurposeExpresses 6xHis-MBP tagged Mb3Cas12aDepositorArticleInserthuMb3Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II SK--POLQ
Plasmid#64873Purposecloning vector for human POLQDepositorInsertPOLQ (POLQ Human)
ExpressionBacterialAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pgaC
Plasmid#154919PurposeaTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pgaC
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpTetAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer
Plasmid#176588PurposeEndogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Delaware Bay) locus
ExpressionBacterialMutationReplaced spacer in CRISPR array with Fn spacerAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-30a-NSP14-SARS-CoV-2
Plasmid#236423Purposebacterial protein expression of NSP14DepositorAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Ufd1-His
Plasmid#117107PurposeProduction of recombinant Ufd1-His in E.coliDepositorAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS0806 His14-bdSUMO-AwaIscB
Plasmid#176539PurposeBacterial expression of His14-bdSUMO-AwaIscBDepositorInsertAwaIscB
UseCRISPRTags14xHis-bdSUMO tagExpressionBacterialPromoterT7Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mEmerald
Plasmid#103982PurposePlasmid encoding mEmeraldDepositorInsertmEmerald
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
TagsP2A-mCherryExpressionBacterialPromoterno promoterAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP3665
Plasmid#222351PurposeExpression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETa_ mEos4b
Plasmid#51073PurposePhotoconverible Fluorescent Protein_ Fixative Resistant (monomeric)DepositorInsertmEos4b
ExpressionBacterialPromoterT7Available SinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG031 (upx)
Plasmid#157909PurposeExpresses the upx defense systemDepositorInsertupx (putative PD-(D/E)xK family phosphodiesterase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD454-SR rat Alpha-synuclein
Plasmid#89074Purposebacterial expression of rat Alpha-synucleinDepositorAvailable SinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits