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32,156 results

Showing: 4981 - 5010 of 32156 results

  1. 6xHis-MBP-huBsCas12a

    Plasmid
    #174671
    Purpose
    Expresses 6xHis-MBP tagged BsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    174671-plasmid-map-sequence-id-364005

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  2. pMO9075

    Plasmid
    #117481
    Purpose
    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR
    Depositor
    Judy Wall
    Article
    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1
    Insert
    Aph(3')-IIa promoter
    Expression
    Bacterial
    Promoter
    Aph(3')-IIa promoter
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    117481-plasmid-map-sequence-id-273023

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  6. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  9. PHYHD1

    Plasmid
    #38854
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHYHD1 (Phyhd1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

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  15. pBAD-FimH-9xHis

    Plasmid
    #97305
    Purpose
    E.coli UTI89 FimH signal peptide and lectin domain
    Depositor
    Per Svenningsen
    Insert
    Type 1 fimbiral adhesin FimH
    Tags
    9xHis
    Expression
    Bacterial
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    97305-plasmid-map-sequence-id-182575

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  17. pUS116

    Plasmid
    #84578
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    84578-plasmid-map-sequence-id-389132

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  18. pCVL.SSA TLR (Sce)

    Plasmid
    #45576
    Purpose
    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Sce I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available Since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45576-plasmid-map-sequence-id-67850

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  20. p404TDH3

    Plasmid
    #15973
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15973-plasmid-map-sequence-id-8458

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  24. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108867-plasmid-map-sequence-id-209739

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Showing: 4981 - 5010 of 32156 results