32,671 results
-
Plasmid#62793PurposeLocalization: Protein Expression Vector , Excitation:N/A, Emission:N/ADepositorTypeEmpty backboneTags6xHis and TagRFPExpressionBacterialMutationnoneAvailable SinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NR
Plasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-2
Plasmid#90326PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694DepositorInsertTetR
ExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
EPHA7 (2REI)
Plasmid#25148PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTarget: Ptaq-RFP_PlacIq-GFP
Plasmid#192280PurposeRFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter PlaciqDepositorInsertsRFP
GFP
ExpressionBacterialPromoterPlacIq and PtaqAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 KD
Plasmid#13322DepositorInsertPINK1 kinase domain (PINK1 Human)
TagsGSTExpressionBacterialMutationAmino acids 112-496; Kinase dead: K219D/D362A/D3…Available SinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNR64
Plasmid#79540PurposeInput plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.DepositorInsertsTP901
BxbI
TagsHis tag and LAA degradation tagExpressionBacterialAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK569
Plasmid#110614PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoterDepositorInsertE2-Crimson
ExpressionBacterialMutationV32M (see comments below)Available SinceOct. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET3a-Ub-K48C
Plasmid#18897DepositorInsertUbiquitin-K48C (UBC Human)
ExpressionBacterialMutationLysine 48 is replaced with a Cysteine residueAvailable SinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
EPB104
Plasmid#68369Purposecontains human U6 promoterDepositorAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
ExpressionBacterialAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-30
Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-sfGFP
Plasmid#117390PurposeTn7 tagging vector pTn7xTS with sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_1
Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
SOCS2A-c016
Plasmid#53689Purposebacterial expression plasmidDepositorInsertSOCS2A
TagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1
Plasmid#136647PurposeExpression of SHBP1-GST fusion protein in E. coliDepositorInsertSH3BP1 (SH3BP1 Human)
ExpressionBacterialAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
B1-5
Plasmid#111467Purposetranslational elongation factor TsDepositorInserttranslational elongation factor Ts
Tags6xHisExpressionBacterialAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
C332
Plasmid#141124PurposeCinI expressing plasmidDepositorAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPro24
Plasmid#17805DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFIP
Plasmid#17565DepositorInsertinversion switch
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdH3proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PARP2A
Plasmid#42450PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPclpBAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB7k-GFP
Plasmid#35360DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet-tns
Plasmid#170628PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
ExpressionBacterialAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDO23A
Plasmid#27367DepositorInsertpUC/ori, bla
UseGateway CloningExpressionBacterialMutationN/AAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAMK2D
Plasmid#38987PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGV3341
Plasmid#170279PurposeExpression of mutant HIV-2 RT p66 reverse transcriptaseDepositorInsertHIV-2 RT p66 (mutant)
ExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk3
Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
TagsHA-6HIS and MKIEEExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK066
Plasmid#65173PurposeEncodes tTDH1 as a Type 4b part to be used in the Dueber YTK systemDepositorInserttTDH1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMF037
Plasmid#157634PurposeBatis maritima MHTDepositorInsertMHT
ExpressionBacterialAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
INPP5EA
Plasmid#42478PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTK570
Plasmid#110615PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoterDepositorInsertE2-Crimson
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD
Plasmid#168153PurposeInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PDCD6IP_ALIX V-domain
Plasmid#178483PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
ELK1_pet28a
Plasmid#131647PurposeBacterial expression of ETS gene ELK1DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
TagsHis-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
TagseGFPExpressionBacterialPromoterT7Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGC4593
Plasmid#110206PurposeAccessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)DepositorInsertsTEV protease
aaRS
ExpressionBacterialPromoterPbad and PtetAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIRT5.1
Plasmid#13738DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
RS35075_pMQ30_KO_Kan
Plasmid#185390PurposeNon-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11DepositorInsertsRR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker
ExpressionBacterialAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(WT)-mCherry
Plasmid#87356PurposePI(WT): Photo-inhibitable/blue light sensitive ; WT: wild typeDepositorAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only