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Plasmid#146446PurposeBacterial Expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
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pOPINK-TRABID (NZF1, aa 1-33)
Plasmid#65437PurposeExpresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coliDepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-33 of TRABID, which …PromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB164
Plasmid#101667PurposeOpto-T7RNAP*(563), half expr.DepositorInsertOpto-T7RNAP*(563), half expr.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMVV094
Plasmid#215302PurposeExpresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone.DepositorInsertsfGFP
UseTagsHisExpressionBacterialMutationPromoterJ23119Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
cp193g7
Plasmid#25950DepositorInsertcp193g7
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDF0044 CjcCas7-11 full locus with synthetic array
Plasmid#172502PurposeEncodes the CjcCas7-11 full locus for bacterial expressionDepositorInsertCjcCas7-11 full locus with synthetic array
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP48-2A(sfGFP)
Plasmid#126993PurposeLevel 2 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH278-GST-TEV-SUP45
Plasmid#29743DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsGSTExpressionBacterialMutationwild typePromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2
Plasmid#124218PurposeCytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2DepositorInsertmNG-sfTq2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT-β3
Plasmid#166579PurposeFor expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.DepositorInsertβ3 (Itgb3 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAP1LC3A
Plasmid#34824DepositorInsertmicrotubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-O-GECO1
Plasmid#113938PurposeBacterial expression of orange fluorescent Ca2+ sensor O-GECO1DepositorInsertO-GECO1
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIS2-TIP47 (Delta 1-190)
Plasmid#17640DepositorInsertTip47 (Delta 1-190) (Plin3 Mouse)
UseTagsHisExpressionBacterialMutationDelta 1-190PromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBbB7c-GFP
Plasmid#35359DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
DT042A
Plasmid#159403PurposeInducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoterDepositorInsertpr.T5-LacO-6xHis-sfGFP
UseTags6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSLK (SLK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 D321N
Plasmid#53202PurposeExpresses GST and HA tagged human ERK2 D321N from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationD321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pET22b-T5-FB-E25TAG
Plasmid#176545PurposeExpresses IgG affinity protein FB with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange glutamic acid 25 to amber stop codonPromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p406MET3
Plasmid#17436DepositorInsertMET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pH6HTC_NAP1L1-Halo
Plasmid#175342PurposeProduction of HaloTag fusion proteinDepositorInsertNAP1L1 (NAP1L1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDE43-MCSq17
Plasmid#27333DepositorInsertqtag17
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pZH521
Plasmid#102674PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1
Plasmid#87737PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL13-MgtC bb118
Plasmid#158982PurposeConstitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.DepositorInsertmgtC
UseTagsExpressionBacterialMutationPromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2Z)
Plasmid#29776DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDB021CH(+)
Plasmid#99209PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; ProK pylT
UseTagsExpressionBacterialMutationPromoterPT7 and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #4
Plasmid#104211PurposeBacterial expression of WW domain #4 from NEDD4DepositorInsertNEDD4 WW domain #4 (NEDD4 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pQIq_MRGS_HIS8_(27G2)_FLAG
Plasmid#199616PurposeEncodes DARPin-FLAG 27G2 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27G2
UseTags8xHis and FLAGExpressionBacterialMutationPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAM17
Plasmid#86473PurposeExpresses p12 and p9DepositorInsertp12, p9
UseSynthetic BiologyTagsnoneExpressionBacterialMutationPromoterpBADAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(276-598)
Plasmid#135437PurposeExpresses SFPQ 276-598 with TEV-cleavable hexahistidine tagDepositorInsertSplicing Factor Proline And Glutamine Rich (SFPQ Human)
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-phaZ
Plasmid#169874PurposeSingle guide RNA plasmid for Cas9 targeting phaZ in P. putidaDepositorInsertsgRNA towards phaZ in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1477
Plasmid#205970PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)DepositorInsertCas5-HNH all proteins, with His-tagged Cas11
UseTags14xHis on Cas11ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZP17 Krox20
Plasmid#16924DepositorInsertKrox20 (egr2b Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72114-BBa_J72100
Plasmid#40781DepositorInsertlacZ
UseSynthetic Biology; PhagemidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2 RanGAP
Plasmid#13379DepositorInsertRanGAP (RANGAP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-RFP
Plasmid#35333DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTrcAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pQTEV-STX6
Plasmid#31581DepositorInsertsyntaxin 6 (STX6 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLmrA-N1
Plasmid#74684PurposeLmrA-N1 gateDepositorInsertPTac-RBSN1-LmrA-PLmrA-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTac-E-luci
Plasmid#65937PurposeTheophylline Riboswitch E with luciferase geneDepositorInsertluciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only