31,059 results
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Plasmid#118498Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmCardinal
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSPPc
Plasmid#116918PurposeGene constitutive expression from a Pc promoterDepositorTypeEmpty backboneExpressionBacterialPromoterPc promoter from Delftia acidovorans C17Available SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mClover3
Plasmid#103994PurposePlasmid encoding mClover3DepositorInsertmClover3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
TagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PTP1B-1-401
Plasmid#78683Purposebacterial expression of PTP1B aa 1-401DepositorAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP trigger
Plasmid#107359PurposeTrigger sequence for pNP1 sfGFP toehold switchDepositorInserttoehold trigger 7
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO AMPKa (11-281)
Plasmid#177851PurposeBacterial Expression of AMPKa1DepositorAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
ACAA1
Plasmid#38847PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆repeats-mNeonGreen-IRESpuro2
Plasmid#183742PurposeExpresses Ki67∆repeats-mNeonGreenDepositorInsertMKI67(lacking repeats) (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking repeats)PromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0750
Plasmid#115511PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.DepositorInsertgusA pOGG083, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15
Plasmid#153523PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#15DepositorInsertSb#15
ExpressionBacterialPromoterpBADAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7RNAP Cyslight V174C
Plasmid#205078PurposeT7RNAP protein expressionDepositorInsertT7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
ExpressionBacterialMutationC125S, C347S, C510S, C515S, C530S, C723S V174CAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
Tags6His-TEVExpressionBacterialAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
Tags6xHis-GSTExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro2GFP
Plasmid#82366PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro2GFP
Tags6xHISExpressionBacterialMutationC48S/S65T/Q80R/S147C/Q204CPromoterT7Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MERTK_HUMAN_D0
Plasmid#79705PurposeThis plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCPP5099
Plasmid#128714PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopK1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5328
Plasmid#128721PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopU1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5306
Plasmid#128712PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopH1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5571
Plasmid#128713PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopI1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5539
Plasmid#128728PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAF1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5618
Plasmid#128709PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopE1
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5617
Plasmid#128706PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopB1
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT91
Plasmid#109305PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tagDepositorTypeEmpty backboneTagsSufI 1-30, 6HisExpressionBacterialPromoterT7Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD10
Plasmid#31332DepositorInsertPSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CDYL
Plasmid#25509PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
NEK2
Plasmid#39163PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCON1-ProC.gfp
Plasmid#112517PurposeProC driven gfpmut3 expression with synTTDepositorInsertProC gfpmut3 TT
ExpressionBacterialPromoterProCAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
Tags6HisExpressionBacterialMutationD16A H588AAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIM1708-surT-PsurP-agaN-pNW33N
Plasmid#44009DepositorInsertsurT-PsurP-agaN
ExpressionBacterialMutationDeleted EcoRI site (synonymous change, C to T at …Available SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
RRAS2
Plasmid#38816PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_sensor_LacZ
Plasmid#139464PurposeT7 RNAP-driven expression of SNIPR RNA with a LacZ reporterDepositorInsertSNIPR_lacZ
ExpressionBacterialAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SIMx6
Plasmid#116938PurposeBacterial expression vector for 6 simDepositorInsert6 sim
ExpressionBacterialPromoterT7Available SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SUMOx10
Plasmid#116937PurposeBacterial expression vector for 10 sumoDepositorInsert10 SUMO
TagsHISExpressionBacterialPromoterT7Available SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3_ mTFP1- Vamp2
Plasmid#201214PurposeAAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminalsDepositorInsertmTFP1 (from addgene #54613)
UseAAVTagsmouse Vamp2ExpressionBacterial and MammalianPromoterTRE3GAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.EcoRI-p15A-aadA
Plasmid#149341Purposeexpresses M.EcoRI DNA methyltransferase in E. coliDepositorInsertM.EcoRI
ExpressionBacterialPromoterrhamnoseAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinC
Plasmid#133628PurposeExpression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE3c
Plasmid#17463Purposemodified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
SBC015866
Plasmid#226280PurposeExpresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.DepositorInserts4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase
ExpressionBacterialAvailable SinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PET28a-cpLumiLuc-Rapamycin sensor
Plasmid#186292PurposeExpression Rapamycin sensor in e coliDepositorInsertcpLumiLuc-Rapamycin sensor
TagsHisTagExpressionBacterialAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only