32,126 results
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Plasmid#174501Purposebacterial co-expression of human SEPT7 and of human SEPT9_i3DepositorAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_41
Plasmid#59964PurposeHigh full length T7 RNAP* expressionDepositorInsertT7* RNAP
ExpressionBacterialMutationN/APromoterPJ23105Available SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Pak1 83-149
Plasmid#12216DepositorInsertPak1 (Pak1 Rat)
TagsGSTExpressionBacterialMutationDominant negative Pak1: autoinhibitory domain, aa…Available SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBHM2617
Plasmid#234496PurposeNATR-CnU6-empty sgRNADepositorInsertNAT-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'
Plasmid#209115PurposeBacterial expression of CC-GEMS ligand SUMO-A'DepositorInsertSUMO-A'
ExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLIP_CAP_Gly
Plasmid#178470PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMyrPalm_mEGFP_IRES_puro2b
Plasmid#21038DepositorInsertpMyrPalm
TagsmEGFPExpressionBacterial and MammalianAvailable SinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsEYFP and SNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI
Plasmid#115220PurposeExpresses Pin3Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin3Cas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyExpressionBacterialPromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU
Plasmid#64180Purposeharbours marker gene URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCF10 HLA-B*08:01
Plasmid#180447PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*08:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_HuD_FL
Plasmid#65751Purposeplasmid for bacterial overexpression of HuDDepositorAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Plac-SO
Plasmid#218358PurposeEmpty vector with lacIq-Plac expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-SF2
Plasmid#99020PurposeBacterial expression of SF2DepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Poliovirus 1 PV1 isolate 09_05_19 3C protease
Plasmid#228647PurposeBacterial expression of the Polio 3C proteaseDepositorInsertPolio virus 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKJ0464
Plasmid#228716PurposeBacterial expression of T7 RNAP with E643G mutation; for protein production and purificationDepositorArticleInsertSUmo_T7pol_E643G
ExpressionBacterialMutationE643GAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AraC/pBad-Tnpase (camR)
Plasmid#213909PurposeArabinose-inducible himar1C9 transposase expression - cam resistanceDepositorInsertAraC/pBad ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH628_pJH_LldR_PlldR-sfGFP.3TAG
Plasmid#163727PurposeLactate-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertlldr gfp.3TAG
ExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA
Plasmid#47069PurposeFX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tagDepositorTypeEmpty backboneTags3C protease site (PreScission site), AVI-tag, and…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
Tags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_34
Plasmid#59960PurposePTac GFP expressionDepositorInsertsfGFP
ExpressionBacterialMutationN/APromoterPTacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pExp-TF
Plasmid#112575PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-TriggerFactor and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-X0
Plasmid#115565PurposeControl vector expressing GFP alone without any consensus repeatsDepositorInsertGreen Fluorescent Protein
ExpressionBacterial and YeastPromoterADH1Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneExpressionBacterialPromotertac promoter from pKK233-2Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW002-PpsbA-DsRed-Express2
Plasmid#111257PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
ExpressionBacterialPromoterPpsbAAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pReporter_4
Plasmid#60565PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_4
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF-BetI
Plasmid#49357DepositorInsertBetI repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(cAMP-1.6)/pRSETB
Plasmid#53591PurposeNano-lantern-based luminescent cAMP indicatorDepositorInsertNano-lantern-based luminescent cAMP indicator
Tags6xHisExpressionBacterialAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only