32,126 results
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Plasmid#156474PurposeBacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tagDepositorInsertNsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXCA3H
Plasmid#47072PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site), Avi-tag, and…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-Tev-Keap1
Plasmid#62456PurposeHis6-halo tagged human Keap1 protein for E.coli expressionDepositorAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b_msfGFP_stag_KS
Plasmid#237630PurposeFor expression of msfGFP_stag_KS in EcoliDepositorInsertstag_KS
TagsmsfGFPExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b_msfGFP_stag_KS(FtoG)
Plasmid#237631PurposeFor expression of msfGFP_stag_KS(FtoG) in EcoliDepositorInsertstag_KS(FtoG)
TagsmsfGFPExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate PRVABC59 NS3 helicase domain
Plasmid#228664PurposeBacterial expression of the Zika virus NS3 helicaseDepositorInsertZika virus NS3 helicase
TagsHis-GST-TEVExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
RoL10X_pET29b
Plasmid#215427PurposeExpression construct for RoL10X gene, codon optimized for expression in E. coliDepositorInsertRoL10X
ExpressionBacterialMutationV209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…Available SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ThcCut1_pET29b
Plasmid#215429PurposeExpression construct for ThcCut1 gene, codon optimized for expression in E. coliDepositorInsertThcCut1
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJOE4847.2
Plasmid#135086Purposerhamnose-inducible expression vectorDepositorInsertMalE-6xHis-Ulp1
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule-Mb haloTyrRS C6
Plasmid#160377PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16
Plasmid#153524PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#16DepositorInsertSb#16
ExpressionBacterialPromoterpBADAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB078d5
Plasmid#79204PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
human PNP non-tagged
Plasmid#64199PurposeExpression of native human PNP (non-tagged)DepositorAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKJ1267 (aka pAC-alphaGal4)
Plasmid#21869DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAM105
Plasmid#86479PurposeExpresses p9-FLAG-FMODepositorInsertp9-FLAG-FMO
UseSynthetic BiologyTagsFLAG and FMOExpressionBacterialPromoterpTetAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRST11B-AS3_4
Plasmid#102789PurposeExpresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNADepositorInsertsExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIOM15
Plasmid#20869DepositorInsertaphA::aadA
ExpressionBacterialAvailable SinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa2-A69T
Plasmid#98578Purposephotoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertpcDronpa2-A69T
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mCherry-FLAG:cat
Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
ExpressionBacterialMutationNAAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC575
Plasmid#127976PurposeAMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertble (bleomycin resistance marker)
UseFungal expressionExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SETDB1
Plasmid#25240PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETDB1, SETDB1_16 (SETDB1 Human)
Tags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionBacterialPromoterT7Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBSKrnaSeph
Plasmid#67506PurposeExpress the tRNALys scaffold with the sephadex aptamer tagDepositorInsertmodified tRNALys with sephadex aptamer
ExpressionBacterialPromoterlppAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1
Plasmid#109382PurposeArabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
ExpressionBacterialAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046920
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pClodANL-FGF4
Plasmid#107874PurposeBacterial expression of FGF4-NanoLucDepositorAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE147
Plasmid#118499Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023
Plasmid#90317PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorArticleInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtet(Tetracycline inducible protein)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneTagsTwin StrepExpressionBacterialPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS Olfactomedin1
Plasmid#19225DepositorInsertOlfactomedin 1 (OLFM1 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pL1M-pMtRC-MtLAP1-Adh-R1
Plasmid#170790PurposeA Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic StudiesDepositorInsertMtLAP1 (LOC11412013 Medicago truncatula)
ExpressionBacterial and PlantPromoterMedtr4g059670(MtRC)Available SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRAU168
Plasmid#86834Purposebacterial expression of anti-CRISPR protein AcrIIA1DepositorInsertacrIIA1
ExpressionBacterialPromoteraraBADAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-Tn5-F
Plasmid#160081PurposeBacterial expression of C-terminally FLAG-tagged Tn5 transposaseDepositorInsertTn5 transposase
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-Etanercept
Plasmid#182420PurposeBacterial expression of mNeongreen-Etanercept fusionDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC-28S15-0X
Plasmid#20316DepositorInsertPsmyc-tetR#28
ExpressionBacterialAvailable SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJUMP45-2A(sfGFP)
Plasmid#126989PurposeLevel 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only