32,126 results
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Plasmid#62565PurposeFlavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-Logic-AND
Plasmid#112237PurposeGFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.DepositorInsertsGFP under control of doubly-repressible promoter
GFP
araC
ExpressionBacterialPromoterAND-gate and pLacIAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-Plac
Plasmid#122634PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDSacI
Plasmid#38255DepositorTypeEmpty backboneTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialPromoterT7Available SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
4RCI
Plasmid#64653PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET28HistevTtTERT(1-516)
Plasmid#28171DepositorInsertTetrahymena TERT
Tags6xhistidineExpressionBacterialAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
YWHAB
Plasmid#39128PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a HAT epitope
Plasmid#129445Purposeexpression of HAT epitopeDepositorInsertHAT epitope
ExpressionBacterialAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
BRDT
Plasmid#38898PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL1-E2C
Plasmid#180421PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.DepositorInsertE2 Crimson
ExpressionBacterialAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
TagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
5T5G (SETD8)
Plasmid#101886PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
poRFP-oGFP
Plasmid#154130PurposeExpresses orthogonal gfp and orthogonal rfp genes in bacterial cellsDepositorInsertsorthogonal gfp
orthogonal rfp
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MRAS
Plasmid#55661Purposeexpression of mouse MRASDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCPP5233
Plasmid#128715PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcM-hopM1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV2_pet28a
Plasmid#131665PurposeBacterial expression of ETS gene ETV2DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB076i5
Plasmid#79199PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2+3); Ppro1Available SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialPromoterTRE tight promoterAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC011 LwCas13a locus with targeting spacer
Plasmid#91903PurposeLwCas13a locus into pACYC184 with targeting spacerDepositorInsertLwCas13a with targeting spacer
UseCRISPRExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSG019
Plasmid#126502PurposePseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHisDepositorInsertdisaggregase ClpG with 6xHis-tag
TagsC-terminal 6xHisExpressionBacterialPromoterPBADAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Full Length
Plasmid#159142PurposeExpresssion and protein purification of full length RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
ExpressionBacterialAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailable SinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)
Plasmid#159698PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_IPF2.0
Plasmid#163124PurposeA plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.DepositorInsertIFP2.0
TagsHisExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
ExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
TagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
V621 pGEX-4T-LP - Acceptor
Plasmid#11715DepositorTypeEmpty backboneUseCre/Lox; Acceptor vectorTagsGSTExpressionBacterialAvailable SinceMay 24, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-30 Ubwt (2)
Plasmid#26685DepositorInsertUbiquitin
TagsHis and S-tagExpressionBacterialAvailable SinceNov. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
ARH1-c003
Plasmid#173093PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only