29,813 results

1. pFCiR5

   Plasmid

   #149479

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL030

   Plasmid

   #53533

   Purpose

   supplies EtpC required for optimal EtpA expression on compatible plasmid

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpC
   

   Use

   Pacyc184

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. R701-X71-527: His6-tev-Hs.CALM1(1-80)

   Plasmid

   #159694

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(1-80)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(1-80) (CALM1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pgex-cIAP1 mut

   Plasmid

   #8334

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   ciap1mut (BIRC2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   H588A

   Promoter

   Available since

   Nov. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

   Plasmid

   #238003

   Purpose

   Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtRBX1 (RBX1 Mustard Weed)
   AtSKP1 (SKP1 Mustard Weed)
   AtCUL1 (CUL1 Mustard Weed)
   

   Use

   Tags

   S and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYO1125

   Plasmid

   #235742

   Purpose

   Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   p40 PX domain (NCF4 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   aa 2-149 with Cys at 2

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF-MjCouRS

   Plasmid

   #229066

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   Methanococcus jannaschii tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGal-Trans-Myc-eGFP-KDEL

   Plasmid

   #177729

   Purpose

   ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
   

   Use

   Tags

   KDEL, Myc, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. R701-X72-527: His6-tev-Hs.CALM1(76-148)

    Plasmid

    #159695

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(76-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(76-148) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHHM.X513-iNicSnFR3b-(V7)

    Plasmid

    #124881

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)
    

    Use

    Tags

    HA Tag, His Tag, and NA

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPSU20-40-60-80

    Plasmid

    #173839

    Purpose

    Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACCBPv3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

    Plasmid

    #199371

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBad-sfGFP 150TAG

    Plasmid

    #85483

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150TAG (amino acid 150 mutated to stop codon)

    Promoter

    Arabinose

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. bZiPro

    Plasmid

    #86846

    Purpose

    express NS2B-NS3 protease in E.coli

    Depositor

    Dahai Luo

    Article

    Zhang et al Science. 2016 Dec 8. pii: aai9309.

    Inserts

    NS3 1-177
    NS2B45-96
    

    Use

    Tags

    a hexahistidine tag and a thrombin cleavage site

    Expression

    Bacterial

    Mutation

    NS2B cofactor region (residues 45–96) and NS3 pro…

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSP3535

    Plasmid

    #46886

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. BRD4

    Plasmid

    #38942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge