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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Publication

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCold TDP43 WT

Plasmid

#27451

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa, GST, and His6

Expression

Bacterial

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Publication

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Tags

His

Expression

Bacterial

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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zbFGF

Plasmid

#12309

Depositor

James Thomson

Publication

Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

Insert

Fgf2 (fgf2 Zebrafish)

Expression

Bacterial

Mutation

Ala 11 mutated to Pro, Ser 55 mutated to Asn

Available since

Sept. 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Tags

C-Tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pTRK892

Plasmid

#71803

Purpose

Expression of mutated L. gasseri gusA from strong Ppgm promoter

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Publication

Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

Insert

gusA3

Expression

Bacterial

Mutation

D573A

Promoter

Ppgm

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Publication

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDB1 (CK2alpha)

Plasmid

#27083

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDuet TDP43 WT

Plasmid

#27462

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

GST

Expression

Bacterial

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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UbcH5c WT pET28a

Plasmid

#12643

Depositor

Rachel Klevit

Publication

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)

Expression

Bacterial

Available since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Publication

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Expression

Bacterial

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET_mNG2(1-10)_32aalinker_mNG2(11)

Plasmid

#82611

Purpose

Bacterial expression vector for split mNeonGreen2 screening

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)

Expression

Bacterial

Mutation

split before mutations

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Publication

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Publication

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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mApple-pBAD

Plasmid

#54536

Purpose

Localization: Protein Expression Vector , Excitation: 568, Emission: 592

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

6xHis and mApple

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCW-LIC

Plasmid

#26098

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

Three consecutive Taq promoters, 2 active

Expression

Bacterial

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Publication

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET15b CnA CnB

Plasmid

#11787

Depositor

Anjana Rao

Publication

Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

Insert

CnA CnB (PPP3R1, PPP3CA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles