32,684 results
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Plasmid#71476Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-D16A-NmeCas9
Tags6HisExpressionBacterialMutationD16AAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
INMT
Plasmid#25475PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PhdeA-cfp
Plasmid#127940PurposeCFP fluorescent reporter for HdeADepositorInsertPhdeA-cfp
ExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSA120
Plasmid#59789PurposeC. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operonDepositorInsertshsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR
ExpressionBacterialAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP6
Plasmid#80662PurposeEncodes the synthetic coiled coil peptide SYNZIP6DepositorInsertSYNZIP6
Tags6xHisExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP5
Plasmid#80661PurposeEncodes the synthetic coiled coil peptide SYNZIP5DepositorInsertSYNZIP5
Tags6xHisExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
Tags6XHisExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-sfGFP
Plasmid#103983PurposePlasmid encoding sfGFPDepositorInsertsfGFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZa-STE2-1D4
Plasmid#133436PurposeMutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/BOA
Plasmid#82041PurposeFor E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)DepositorInsertBurkholderia oklahomensis agglutinin
Tags6x HisExpressionBacterialAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2207 pGEM p53
Plasmid#10853DepositorInsertp53 (TP53 Human)
ExpressionBacterialAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-EsaI
Plasmid#47660PurposeesaI gene downstream of esaR in pAC-EsaR (CmR)DepositorInsertesaI-term
UseSynthetic BiologyExpressionBacterialPromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-BRIC
Plasmid#172343PurposeBioluminescent Red Indicator for Calcium (bacterial expression)DepositorInsertBRIC
ExpressionBacterialAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZP17 Krox20
Plasmid#16924DepositorInsertKrox20 (egr2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
6ASB (FBXL19)
Plasmid#119954PurposeBacterial expression for structure determinationDepositorInsertFBXL19 (FBXL19 Human)
Tags6xHis-TEVExpressionBacterialMutationcontains amino acids 31-153PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
hHpr1-GST
Plasmid#11200DepositorAvailable SinceJan. 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
TagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23a-Mm/HsUBA52
Plasmid#69560PurposeBacterial expression; in vitro transcription/translation (T7)DepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
XNOR-gate-V2.0
Plasmid#44454DepositorInsertXNOR-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
Plasmid#83484PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).DepositorInsertLbu_C2c2_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1048A, H1053AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_10
Plasmid#60581Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_10
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
ExpressionBacterialPromoterlacAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbK
Plasmid#114152PurposeExpresses ClbKDepositorInsertclbK
Tags6xhis-ThrombinExpressionBacterialAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbI
Plasmid#114151PurposeExpresses ClbIDepositorInsertclbI
Tags6xHisExpressionBacterialAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbF
Plasmid#114148PurposeExpresses ClbF-cHisDepositorInsertClbF
Tags6xHisExpressionBacterialAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbC
Plasmid#114145PurposeExpresses ClbC-nHisDepositorInsertclbC
Tags6xHis-thrombin-T7 tagExpressionBacterialAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbE
Plasmid#114147PurposeExpresses ClbE-cHisDepositorInsertClbE
Tags6xHisExpressionBacterialAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbD
Plasmid#114146PurposeExpresses ClbD-cHisDepositorInsertClbD
Tags6xHisExpressionBacterialAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbG
Plasmid#114150PurposeExpresses ClbGDepositorInsertclbG
Tags6xHisExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
Tags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180153Purposetd-sfGFP dre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_1
Plasmid#109233PurposeEmpty AmpR pSC101 vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ETV6-ETS
Plasmid#111180PurposeMinimal ETS domain of human ETV6 (TEL)DepositorAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2 + sfGFP
Plasmid#88918PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HaloGFP-Na2.4
Plasmid#228470PurposeBacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4DepositorInsertHaloGFP-Na2.4
ExpressionBacterialAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS1: pHelper(AvCAST)_AvPSP1
Plasmid#168133PurposeInducible expression of AvCAST proteins with crRNA targeting AvPSP1.DepositorInsertsAvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS ColI alpha2
Plasmid#19207DepositorInsertAlpha 2 type I collagen (COL1A2 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
NMT1
Plasmid#25274PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29b-clbBpks
Plasmid#114154PurposeExpresses the PKS module of ClbBDepositorInsertclbB
Tags6xHisExpressionBacterialMutationOnly contains the PKS module of ClbBAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
Tags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-Available SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046740
Plasmid#145588PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
U_(+36GFP)-BirA
Plasmid#71753PurposeU+36GFP-BirA for expression in E.ColiDepositorInsertU_(pos36GFP)_BirA_6XHis_LPETG
Tags6XHisExpressionBacterialAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC301
Plasmid#31851DepositorInsertGFP (including ATG start codon)
ExpressionBacterialPromoterSODAvailable SinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBAD*RFPEC2
Plasmid#17823DepositorInsertE. coli optimized red flourescent protein (RFPec2)
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ209
Plasmid#162682PurposeLidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
TagsHisExpressionBacterialMutationReplacement of G251-T472 with W185-F191 from PETa…PromoterT7/lacAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
KSP_K560GFP
Plasmid#129770PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKSP (kif11.L Frog)
TagsGFP and His6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…Available SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only