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Showing: 10701 - 10750 of 29646 results

  5. pGEX-MK1

    Plasmid
    #29638
    Depositor
    Bruce Tempel
    Insert
    Mk1 (Kcna1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  8. pTXB1-CI

    Plasmid
    #51565
    Purpose
    Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cI
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51565-plasmid-map-sequence-id-78820

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  10. SETD2

    Plasmid
    #25348
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD2 (SETD2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. SETD3

    Plasmid
    #32873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD3 (SETD3 Human)
    Use
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. TIF1A

    Plasmid
    #74647
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TIF1A (TRIM24 Human)
    Use
    Tags
    His-6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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  22. RECQL

    Plasmid
    #38890
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RECQL (RECQL Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    S611G
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38890-plasmid-map-sequence-id-178339

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  28. pPbuCas13b-gRNA-NT

    Plasmid
    #184568
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184568-plasmid-map-sequence-id-374001

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  30. SCLYA

    Plasmid
    #42358
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SCLYA (SCLY Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  41. pCRISP_IS

    Plasmid
    #120425
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120425-plasmid-map-sequence-id-240993

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  43. TBXTA-c027

    Plasmid
    #139764
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Use
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139764-plasmid-map-sequence-id-296577

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  46. pHybr_r2a>mG1-srt-his

    Plasmid
    #124802
    Purpose
    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG1
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    -
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124802-plasmid-map-sequence-id-244317

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  50. pET22-6His-SUMO-Nsp10

    Plasmid
    #154960
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154960-plasmid-map-sequence-id-306350

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Showing: 10701 - 10750 of 29646 results