32,684 results
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Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILxR5
Plasmid#149497PurposeBroad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAH5
Plasmid#41829PurposeUsed as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7,TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CSK-1/1
Plasmid#91542PurposeProtein expression and purification of human SH3 domain construct CSK-1/1DepositorInsertCSK-1/1 (CSK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
25 pCOLI_S_NoTag
Plasmid#160261PurposeUntagged Spec resistant bacterial expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAM17
Plasmid#86473PurposeExpresses p12 and p9DepositorInsertp12, p9
UseSynthetic BiologyTagsnoneExpressionBacterialPromoterpBADAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB355
Plasmid#22070DepositorTypeEmpty backboneTagsT18ExpressionBacterialAvailable SinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXY1
Plasmid#90487PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
ExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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B7-4
Plasmid#111492PurposeMet-tRNA formyltransferaseDepositorInsertMet-tRNA formyltransferase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
ExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXA10 (human) HIS-tag pET
Plasmid#8516DepositorInsertHOXA10 (HOXA10 Human)
ExpressionBacterialAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-C125G
Plasmid#174792PurposeBacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
STK24_HUMAN_D0
Plasmid#79742PurposeThis plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB11-PTBP1-RRM2CCtoSS
Plasmid#89154Purposerecombinant expression of PTBP1-RRM2 C250S,C251S in e.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
TagsIntein and chitin binding domainExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2H5
Plasmid#71715PurposeMembrane scaffold protein (MSP), helix 4/2 + helix5 deletionDepositorAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Dengue2 NS2B-NS3 proteins
Plasmid#204792PurposeBacterial expression of a fusion of Dengue2 NS2B-NS3 proteinsDepositorInsertDengue2 NS2B-NS3 fusion
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pM
Plasmid#64179Purposeharbours marker gene MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAV-A
Plasmid#65927PurposeTheophylline Riboswitch A with lacZ geneDepositorInsertlacZ
ExpressionBacterialPromoterT5Available SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag dPDZ-PTEN (1-400)
Plasmid#22232DepositorInsertPTEN (Pten Mouse)
TagsFlagExpressionBacterial and MammalianMutationPDZ binding domain deleted. Contains amino acids …Available SinceMarch 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
TagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C2-77 pComb3xss anti-cFos nanobody
Plasmid#145837PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C2-77 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
6MIW
Plasmid#127291PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorAvailable SinceJune 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS17: pTwinStrep-SUMO-AvTnsD
Plasmid#168150PurposeAvTnsD expression construct for protein purification.DepositorInsertAvCAST TnsD
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS14: pTwinStrep-SUMO-AvTnsA
Plasmid#168147PurposeAvTnsA expression construct for protein purification.DepositorInsertAvCAST TnsA
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-3
Plasmid#12995DepositorInsertTSP-3 (THBS3 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xMyc
Plasmid#129569PurposeEmpty backbone for Myc TagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR196MAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationD193EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
Tags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA13c-RFP
Plasmid#126098PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23102Available SinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c000
Plasmid#175480PurposeProtein expression in bacterial cells. Entire CDS, M1-V288.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLtR5
Plasmid#167505PurposeBroad-Host range expression vector, RSF1010 origin, TetR repressor, PLtetO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertTetR-RFP
UseSynthetic BiologyExpressionBacterialPromoterPLtetO-1Available SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSS2 (hS100B)
Plasmid#26774DepositorInsertS100B (S100B Human)
ExpressionBacterialAvailable SinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
Tags6xHis and FlagExpressionBacterialPromoterT7Available SinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
KaiA
Plasmid#42497DepositorInsertKai A
TagsGSTExpressionBacterialPromotert7Available SinceFeb. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV105
Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
UGDH
Plasmid#38900PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBetI-E1
Plasmid#74677PurposeBetI-E1 gateDepositorInsertPTac-RBSE1-BetI-PBetI-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMCSG28-SH3-588
Plasmid#185920PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferaseDepositorInsertSH3-588
Tags6x HIS tagExpressionBacterialMutationDesigned enzyme with novel interface between huma…PromoterT7Available SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZL2372
Plasmid#48683PurposepET29a_HHH CODA HIV integrase with C-terminal HIS tagDepositorInsertHIV Integrase
TagsHisExpressionBacterialAvailable SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…ExpressionBacterialPromoterEscherichia coli lacIN promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15
Plasmid#226350PurposeExpression of proteasome substrate with tail for molecular rulerDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only