Skip to main content
Addgene
Showing: 10851 - 10900 of 29646 results

  7. PTPRN2

    Plasmid
    #38891
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRN2 (PTPRN2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pMTP130 (pTA106_TnsABC(Tn6900))

    Plasmid
    #164261
    Purpose
    Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.
    Depositor
    Joseph Peters
    Article
    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31
    Insert
    tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    (tnsA) Changed Alanine 125 to Aspartic acid
    Promoter
    Lactose
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    164261-plasmid-map-sequence-id-322777

    Enlarge

  16. PTPN3

    Plasmid
    #38966
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN3 (PTPN3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pAL612

    Plasmid
    #119860
    Purpose
    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysterine 290 to Serine and Glycine 244 to Cystei…
    Promoter
    lac
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119860-plasmid-map-sequence-id-232053

    Enlarge

  19. pST50Trc1-DHFRHIS

    Plasmid
    #63949
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63949-plasmid-map-sequence-id-177355

    Enlarge

  27. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162684-plasmid-map-sequence-id-321526

    Enlarge

  39. UBC53

    Plasmid
    #25471
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc53.001.150 (UBE2U Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. pET24a-VHH-tev-APEX2

    Plasmid
    #117751
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117751-plasmid-map-sequence-id-228349

    Enlarge

  46. ENO3

    Plasmid
    #39072
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ENO3 (ENO3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. pUltra-5HTP

    Plasmid
    #178042
    Purpose
    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T107C, P254T, C255A
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178042-plasmid-map-sequence-id-352270

    Enlarge

Showing: 10851 - 10900 of 29646 results