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32,156 results

Showing: 9201 - 9240 of 32156 results

  6. pDB038b

    Plasmid
    #99212
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    Expression
    Bacterial
    Mutation
    amber codons at positions 29, 83, and 184 of gIII
    Promoter
    Ppsp and PproK
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99212-plasmid-map-sequence-id-192366

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  8. pDest-557

    Plasmid
    #161890
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coli
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    MBP
    Expression
    Bacterial
    Available Since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161890-plasmid-map-sequence-id-322136

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  12. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessinā€¦
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  14. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31ā€“412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-Bā€¦
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  19. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pJMP8930

    Plasmid
    #220905
    Purpose
    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220905-plasmid-map-sequence-id-436985

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  33. pAGH42

    Plasmid
    #107249
    Purpose
    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    vipp1-gfp
    Use
    Suicide vector for destined for double homologousā€¦
    Tags
    mGFPmut3
    Expression
    Bacterial
    Promoter
    native promoter of sll0617
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107249-plasmid-map-sequence-id-207556

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  35. 5VC9 (CXXC4)

    Plasmid
    #101263
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC4 (CXXC4 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101263-plasmid-map-sequence-id-197652

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  40. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9201 - 9240 of 32156 results