31,059 results
-
Plasmid#175469PurposeProtein expression in bacterial cells. FERM domain, M1-E346. Can be biotinylated.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only
-
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbA
Plasmid#112562PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-DsbA(AA20-208) and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
RGS1
Plasmid#39143PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDF-SeAvs1
Plasmid#188803PurposeCodon optimized SeAvs1 with C-terminal His tagDepositorInsertSeAvs1
TagsHisExpressionBacterialPromoterT7Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCYP15
Plasmid#212848PurposeExpresses the Micromonospora cremea LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP03
Plasmid#212836PurposeExpresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
ExpressionBacterialPromoterLac (proteins), pJ23119 (crRNA)Available SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCE120
Plasmid#204491PurposeFigure 1. reporter circuitDepositorInsertsEBFP2
mKate2
EYFP
UseSynthetic BiologyTagsnoneExpressionBacterial and MammalianPromoterhEF1aAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_mScarlet-I
Plasmid#204110PurposeType 0 CDS partDepositorInsertmScarlet-I red fluorescent protein
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD26
Plasmid#208273PurposeProduction of levopimaradiene in E. coli MEV20DepositorInsertstHMGR
LPS
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD12
Plasmid#208264PurposeProduction of miltiradiene in E. coli MEV20DepositorInsertstHMGR
cldB
smKAL
ExpressionBacterialAvailable SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SmtA Synechococcus elongatus PCC 7942 Metallothionein
Plasmid#200298PurposeBacterial expression plasmids for production of recombinant Synechococcus elongatus PCC 7942 MetallothioneinDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein (SYNPCC7942_RS13750 Synechococcus elongatus)
TagsChiting Binding Domanin-InteinExpressionBacterialAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP23
Plasmid#199372PurposeEncodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJMH147
Plasmid#186647PurposeaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilADepositorArticleInsertaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP579
Plasmid#159598PurposeExpress Cba and Cbi proteinDepositorInsertcba and cbi
ExpressionBacterialMutationnoneAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sh3bp2-SH2
Plasmid#46484DepositorInsertSh3bp2 (SH3BP2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-561PromoterTacAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY7
Plasmid#21766DepositorInsertECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats
TagsHisExpressionBacterialAvailable SinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
TOPO EFS
Plasmid#68451PurposeTOPO construct expressing EFS for generation of GMAP-compatible promoterDepositorInsertElongation Factor 1α Promoter
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRb03_up1198
Plasmid#49706DepositorInsertsfgfp
UseSynthetic BiologyExpressionBacterialPromoterPecf03_up1198Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-ZFN_L-HiBiT
Plasmid#198319PurposeLoads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domainDepositorInsertPdp1_NTD-ZFN_L-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AURKB_Pkinase
Plasmid#110025PurposeProtein expression and purification of AURKB_PkinaseDepositorInsertAURKB_Pkinase (AURKB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
TagsHis10ExpressionBacterialPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-DJ1-WT
Plasmid#29350DepositorAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKEW215_AcrIIA13
Plasmid#138281PurposeExpression of AcrIIA13 proteinDepositorInsertAcrIIA13
UseCRISPRTagsMBPExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4A6
Plasmid#73434PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.DepositorInsertRepressor 4A6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTPN18
Plasmid#39168PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDM_noro_A2_lacZ
Plasmid#118820PurposeT7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ reporter.DepositorInsertNorovirus toehold switch A2
ExpressionBacterialAvailable SinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TN-XXL pRSETB
Plasmid#45796PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
Tags6xHIS, ECFP, and YFPExpressionBacterialPromoterT7Available SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pGEX-4T-1-3xAU1
Plasmid#129572PurposeEmpty backbone for AU1 tagDepositorTypeEmpty backboneTags3xAU1 and GSTExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TEV-12His
Plasmid#137032Purposeempty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)DepositorTypeEmpty backboneTagsHis12 (TEV protease cleavable)ExpressionBacterialPromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046789
Plasmid#145660PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
TagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-EmGFP-15b
Plasmid#138280PurposeExpress tTALE-EmGFPDepositorInsert7-mer tTALE-EmGFP
UseTALENTags6x His-tagExpressionBacterialPromoterT7 promoterAvailable SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXC3H
Plasmid#47068PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2 SHP2 E76A
Plasmid#8324DepositorAvailable SinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUdO4t
Plasmid#210249Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
TagsHis tag with TEV cleavageExpressionBacterialPromoterT7Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK043
Plasmid#171315PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SF3B1-NTerm
Plasmid#97425PurposeExpresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coliDepositorInsertSF3B1-N-term (AA1-500)
TagsGSTExpressionBacterialMutationSilent mutation at nt876, aa289 (AGG to AGA)PromotertacAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
ExpressionBacterial and YeastPromoterTDH3Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC201
Plasmid#129387PurposeA variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.DepositorInsertoriR6K and oriT
ExpressionBacterialMutationN/APromoterN/AAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)
Plasmid#48292DepositorTypeEmpty backboneTagsHis6 and ThioredoxinExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only