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Showing: 11651 - 11700 of 29656 results

  1. T4 Lysozyme T21C T142C WT*

    Plasmid
    #18244
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T21C T142C WT*
    Promoter
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  3. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

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  12. pMS6: pHelper(AvCAST)_entry_ΔTnsD

    Plasmid
    #168139
    Purpose
    Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168139-plasmid-map-sequence-id-330862

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  17. L1-10XQUAS-R5

    Plasmid
    #41435
    Depositor
    Steve Stowers
    Insert
    10XQUAS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41435-plasmid-map-sequence-id-55245

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  22. HSA9761

    Plasmid
    #25486
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HSA9761 (DIMT1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. CUL3A-c013

    Plasmid
    #53672
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    CUL3A
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    solubilizing mutations I342R & L346D
    Promoter
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pAAH6

    Plasmid
    #41830
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7,TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41830-plasmid-map-sequence-id-69212

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  30. PHPT1A

    Plasmid
    #42360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHPT1A (PHPT1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  37. RRAS

    Plasmid
    #38815
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RRAS (Rras Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pTMS1

    Plasmid
    #87799
    Purpose
    For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87799-plasmid-map-sequence-id-177269

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  42. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131774-plasmid-map-sequence-id-258731

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  44. pGMC00018 (aka pRC0082)

    Plasmid
    #195320
    Purpose
    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195320-plasmid-map-sequence-id-387102

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  45. human KANK1 Ankyrin Repeats pET151

    Plasmid
    #164053
    Purpose
    Expresses human KANK1 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164053-plasmid-map-sequence-id-327672

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Showing: 11651 - 11700 of 29656 results