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pE-SUMO CRYGS
Plasmid#80753PurposeExpression of human gamma S-crystallin in E. coliDepositorInsertCRYGS (CRYGS Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET 15b-UbcH5c/2418
Plasmid#11473DepositorInsertUbcH5c (UBE2D3 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP682-1
Plasmid#70050PurposeExpresses TEV::eGFP::myc::3Xflag in bacteriaDepositorInserteGFP
UseTagsTEV and myc::3XflagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBluescript alpha-SSeCKS
Plasmid#61295Purposecontains rat alpha SSeCKS cDNADepositorInsertalpha SSeCKS (Akap12 Rat)
UseTagsExpressionBacterialMutationcDNA with 1kb of 3' UTR and 150bp of 5'…PromoterT7Available sinceDec. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-NEMOL329P
Plasmid#11967DepositorInsertNEMOL329P (IKBKG Human)
UseTagsGSTExpressionBacterialMutationLeucine329 to ProlinePromoterAvailable sinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pET21-tVYN-Nt∆
Plasmid#154431PurposePlasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detectionDepositorInsertVenusΔC12-NtYcgRΔ-NLucΔ4
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK039
Plasmid#171311PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertpyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK084
Plasmid#171356PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Met] A.niger Pol-III terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS cTbx3 (CT#23)
Plasmid#13920DepositorInsertTbx3 (TBX3 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
p16-1 hTRF2
Plasmid#12299DepositorInserthTRF2 (TERF2 Human)
UseTagsExpressionBacterialMutationA434 deleted; There is a stop codon in the 5'…PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pFA-sbtA
Plasmid#133011PurposepFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27)DepositorInsertSbtA
UseTags6xhisExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBB3
Plasmid#80750PurposeExpression of human beta B3-crystallin in E. coliDepositorInsertCRYBB3 (CRYBB3 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-Ty1
Plasmid#194588PurposeBacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertTy1 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-MBP-TEV-Cas2
Plasmid#186275PurposeExpresses Thiomicrospira Cas2 in E. coliDepositorInsertThiomicropira Cas2
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PARD6A_PDZ_1
Plasmid#103946PurposeProtein expression and purification of TAX INTERACTION PROTEIN PDZ domainDepositorInsertPARD6A (PARD6A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript SK - hTRF1
Plasmid#12306DepositorInserthTRF1 (TERF1P3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBR322-bom
Plasmid#155178PurposepBICI_2_strong without bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Na0.5
Plasmid#198634PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-Na0.5
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD_GFP_mCherry_++
Plasmid#187391Purposearabinose-inducible expression of GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCx35IL-pET42
Plasmid#42916DepositorInsertConnexin 35 cytoplasmic loop
UseTags6x His, GST, and S-tagExpressionBacterialMutationcytoplasmic loop is aa 101-179PromoterT7Available sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 CCAAA
Plasmid#98402PurposeBacterial expression vector for Trx1 CCAAADepositorInsertTrx1 CCAAA (TXN Human)
UseTagsExpressionBacterialMutationC62A, C69A, C73APromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 SSAAA
Plasmid#98403PurposeBacterial expression vector for Trx1 SSAAADepositorInsertTrx1 SSAAA (TXN Human)
UseTagsExpressionBacterialMutationC32S, C35S, C62A, C69A, C73APromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SETMAR (3BO5)
Plasmid#25250PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETMAR_11 (SETMAR Human)
UseTagsHisExpressionBacterialMutationHistone-lysine N-methyltransferase domain: Residu…PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p53-(1-393)-E285K-N268D
Plasmid#35089DepositorInsertp53 (TP53 Human)
UseTagsHis and Thrombin cleavage siteExpressionBacterialMutationContains aa 1-393 with N269D and E285K mutationsPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJKW0826
Plasmid#119027PurposeE.coli expression plasmid for PmKOMT2DepositorInsertPmKOMT2
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoformPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS chick IRX4 (CC#7)
Plasmid#15139DepositorInsertIrx4 (IRX4 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS/KS-mId2sp
Plasmid#16044DepositorInsertId2 (Id2 Mouse)
UseTagsExpressionBacterialMutationcDNA nucleotides 1-195PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tagDepositorInsertS-Luc_CLIP
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4A
Plasmid#145366PurposeCell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherryDepositorInsertZIKV NS4A
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
proISG15 C-term. domain (79-165)
Plasmid#110763PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3_HMV-His6
Plasmid#164843PurposeExpression of FL human metavinculinDepositorInsertVCL (VCL Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-PITPbeta (splice variant 1)
Plasmid#58270Purposebacterial expression of PITP beta (phosphatidylinositol transfer protein beta)DepositorInsertPITP beta (PITPNB Human)
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-human RNF146 RING-WWE (30-183)
Plasmid#132611Purposeexpresses human RNF146 RING-WWE (30-183) in E. coliDepositorInsertRNF146 RING-WWE (30–183)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
RBFOX3_RRM_pGEX
Plasmid#135131PurposeRecombinant expression of RBFOX3 with dual-affinity tagDepositorInsertRBFOX3 (RBFOX3 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceMarch 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV co-transformation cloning vector (13S-R)
Plasmid#48328DepositorTypeEmpty backboneUseTagsStrepIIExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dom34(full length)
Plasmid#19098DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RPA1_RepA-N
Plasmid#110032PurposeProtein expression and purification of RPA1_RepA-NDepositorInsertRPA1_RepA-N (RPA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf10
Plasmid#156486PurposeBacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tagDepositorInsertOrf10 (ORF10 Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-144
Plasmid#118528PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CBX3 (3DM1)
Plasmid#25242PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCBX3_05 (CBX3 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-APIC-LexAGAD
Plasmid#112807Purposegateway cloning destination vector to express enhancer driven LexAGAD in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK080
Plasmid#171352PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTcyc1 YJR048W terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK079
Plasmid#171351PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertToat1 Pc18g03600 terminator
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only
