32,167 results
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Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL030
Plasmid#53533Purposesupplies EtpC required for optimal EtpA expression on compatible plasmidDepositorInsertetpC
UsePacyc184ExpressionBacterialAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
ExpressionBacterialMutationNOAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
AK5
Plasmid#39136PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 WT
Plasmid#67607Purposebacterial expression of wild type human PRMT9DepositorAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBL4
Plasmid#50548PurposeExpresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG22-pBAD-TetR
Plasmid#102451PurposeExpresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-eyfp
Plasmid#65039PurposeZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-Plac
Plasmid#122632PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPro18
Plasmid#17806DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
ExpressionBacterialAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLC-255
Plasmid#124292PurposeExpression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-134
Plasmid#118520PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-irp3 WT
Plasmid#87106Purposeexpresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocoliticaDepositorInsertirp3 WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbB1a-GFP
Plasmid#35340DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterTrcAvailable SinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
hsp70:Arl13b-mKate2
Plasmid#105964Purposeheat shock inducible transgenesis, primary cillium markerDepositorAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF53a
Plasmid#17879DepositorInsertBAF53a (ACTL6A Human)
ExpressionBacterialAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-Venus-pBluescript
Plasmid#166756PurposeFor purification of Venus fluorophoreDepositorInsertVenus
Tags6x Histidine tag, and TEV protease siteExpressionBacterialPromoterT7Available SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_hGH
Plasmid#182410PurposeBacterial expression of human growth hormoneDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJ208
Plasmid#162681PurposeLidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
TagsHisExpressionBacterialMutationReplacement of W185-F191 with G251-T472 from MHET…PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-meGFP-DLC1
Plasmid#192048PurposePlasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFPDepositorInsertDLC1 (DLC1 Human)
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-Rab27a
Plasmid#194985Purposeoverexpress Rab27aDepositorInsertGFP-Rab27a
ExpressionBacterialAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pELlR4
Plasmid#172545PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SPA2G231V
Plasmid#42079DepositorInsertSurfactant Protein -A2 (SFTPA2 Human)
UseLentiviral; PlentiTagsV5ExpressionBacterial and MammalianMutationChanged Glycine 231 to ValinePromoterCMVAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only