32,812 results

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pGEX SHP-1(NC)-SH2

Plasmid

#46496

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(NC) (PTPN6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-221

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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p-spTorA-GFP-H6C

Plasmid

#168517

Purpose

GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

spTorA-GFP-H6C

Tags

6xHisC tag and spTorA (signal peptide of E. coli …

Expression

Bacterial

Mutation

The mut3 GFP variant with 5 additional mutations …

Promoter

araBAD

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mNeonGreen

Plasmid

#191743

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mNeonGreen

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1200

Plasmid

#138526

Purpose

For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

Depositor

Gira Bhabha, Damian Ekiert

Article

Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

Insert

mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)

Tags

N-terminal His tag and TEV cleavage site on MlaD

Expression

Bacterial

Promoter

araBAD

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2076.2_TS

Plasmid

#140667

Purpose

Toggle switch (TS)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Toggle switch (TS)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.419

Plasmid

#187208

Purpose

Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco4 ncRNA
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBlu-CGT-1

Plasmid

#170158

Purpose

Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

Depositor

Sarah MacDonald

Insert

Cyclomaltodextrin Glucanotransferase

Expression

Bacterial

Promoter

Tac

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Promoter

ptac

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPA1-Halo

Plasmid

#175307

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T HHARI RBR

Plasmid

#31254

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Human Homologue of Ariadne (HHARI) (ARIH1 Human)

Tags

GST

Expression

Bacterial

Mutation

RBR domain residues 177-395

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCAT207

Plasmid

#134884

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b/EYFP-GgVcl 884-1066-ECFP FRET

Plasmid

#46179

Depositor

Susan Craig

Insert

Vcl 884-1066 (VCL Chicken)

Tags

ECFP, EYFP, and His

Expression

Bacterial

Mutation

aa 884-1066 (Vt, tail domain)

Promoter

T7

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-jAspSnFR3

Plasmid

#203458

Purpose

jAspSnFR3 in T7 bacterial expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

Plasmid

#114069

Purpose

T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized AsCas12a with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p_SNIPR_sensor_GFP

Plasmid

#139463

Purpose

T7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporter

Depositor

Alexander Green

Article

Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

Insert

SNIPR_GFP

Expression

Bacterial

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Plasmid

#159580

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-Vps4

Plasmid

#21495

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS4 (VPS4 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

Plasmid

#48313

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGTvL1-SGC

Plasmid

#39188

Purpose

SGC Empty backbone for bacterial expression

Depositor

Nicola Burgess-Brown

Type

Empty backbone

Tags

GST and TEV cleavage site

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pSB1A3_GFP_dT34

Plasmid

#89669

Purpose

expression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT34

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHBFD1Bad

Plasmid

#53564

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSKB3.MPN348

Plasmid

#11437

Depositor

Sung-Hou Kim

Article

Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

Insert

methenyltetrahydrofolate synthetase

Tags

6x His and TEV cleavage site

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pMSC226

Plasmid

#216375

Purpose

xhNup358-Nb2t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup358-Nb2t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TAA

Plasmid

#208187

Purpose

nonsense mistranslation, TAA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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32,812 results