31,059 results
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Plasmid#139766PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
TagsAvi-tag (Biotin) and His6-TEVExpressionBacterialMutationContains only amino acids E41- D225PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-PPARdelta
Plasmid#16548DepositorInsertPPAR delta (PPARD Human)
TagsGSTExpressionBacterialMutationN-terminal DNA-binding domain: aa 1-249Available SinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneTagsHis10 Tag - 3C cleavage siteExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE153
Plasmid#118503Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
Tags6xhis-tag and MBPExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-A21
Plasmid#90318PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXS-sfGFP
Plasmid#117386PurposepGEN-mcs with a modular sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU1105
Plasmid#14469DepositorInsertDsRedT3
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNIDR
Plasmid#157868Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDRDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD2_miniluc_newP7
Plasmid#174105Purposeadding the mini luciferase and mini promoter to the MPRA lenti constructDepositorInsertmini luciferase mini-promoter
ExpressionBacterialAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
plYJP067-Ptet
Plasmid#202299PurposePTet-YFP plasmid for LPattB2 integrationDepositorInsertPtet-YFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
ExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS SOHo1 (CC#99)
Plasmid#15132DepositorInsertSOHo1 (SOHO-1 Chicken)
ExpressionBacterialAvailable SinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXY1-spc
Plasmid#90488PurposePlasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B
Plasmid#67334PurposehArl8b with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-PheB
Plasmid#71738PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertPheB
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
cp193g7
Plasmid#25950DepositorInsertcp193g7
TagsHisExpressionBacterialAvailable SinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11
Plasmid#184277PurposeExpresses nanobody H11 in E. coliDepositorInsertNanobody H11
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA13a-RFP
Plasmid#126086PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23102Available SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLZL2372
Plasmid#48683PurposepET29a_HHH CODA HIV integrase with C-terminal HIS tagDepositorInsertHIV Integrase
TagsHisExpressionBacterialAvailable SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-1
Plasmid#134800PurposeIPTG inducible expression of S-TAGA qtRNADepositorInsertS-TAGA qtRNA
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-WT
Plasmid#134804PurposeIPTG inducible expression of WT S tRNA scaffold tRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ETV1A-c014
Plasmid#53655Purposebacterial expression plasmidDepositorInsertETV1A
ExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C2
Plasmid#50354PurposeMouse eIF2C2 cDNA cloneDepositorAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWarf(-)
Plasmid#34561DepositorInsertHRV 3C-GFP-8His
ExpressionBacterialAvailable SinceJan. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
ARF1
Plasmid#28168PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
Plasmid#176589PurposeEndogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Chesapeake Bay) locus
ExpressionBacterialMutationTruncated CRISPR array to two direct repeats, rep…Available SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB2H2*
Plasmid#163834PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)DepositorInsertSpecR
UseSynthetic BiologyExpressionBacterialMutationTCPTP R222MPromoterPro1;ProD;pLacZ-OptAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PUM2
Plasmid#120385PurposeBacterial expression of MBP-Pumillio2 fusion proteinDepositorAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Antp
Plasmid#86866PurposeBacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.DepositorInsertLinker-Antp
TagsAntpExpressionBacterialAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH18
Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-REP
Plasmid#64966Purposemini-Tn7 vector with oriR6K, for attTn7 site determinationDepositorInsertoriR6K
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCOLA kiwi sensor sfGFP
Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK14_HUMAN_D0
Plasmid#79680PurposeThis plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK14
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
Tagspolyhistidine tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP72-deltaFlincG
Plasmid#19733DepositorAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only