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31,059 results

Showing: 501 - 520 of 31059 results

  4. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  5. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Tags
    His8-Sumo
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

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  8. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-473446

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  10. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87856-plasmid-map-sequence-id-175019

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  11. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11518-plasmid-map-sequence-id-181065

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  12. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Rodolphe Barrangou, Todd Klaenhammer
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Expression
    Bacterial
    Available Since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

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Showing: 501 - 520 of 31059 results