32,164 results
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Plasmid#111823PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 349-369
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AHCYL1A
Plasmid#42465PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK4beta
Plasmid#15517DepositorAvailable SinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
USP08 (3N3K)
Plasmid#28115PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP08 (USP8 Human)
TagsHis tag with TEV siteExpressionBacterialMutationCatalytic domain only.Available SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSW002-Pc-TorA(sp)
Plasmid#111253PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptideDepositorInsertTorA signal peptide
ExpressionBacterialPromoterPcAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
Tags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-UBC-9
Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoofy22
Plasmid#43992DepositorTypeEmpty backboneTagsHis10 and SExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-T172A-P-R
Plasmid#189854PurposeCre-inducible activation of cat-T172A for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-catT172A
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP (T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
Plasmid#169168PurposeConjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.DepositorInsertMAP1LC3B (MAP1LC3B Human)
Tags6X Histidine Tag, TEV cleavage site, mCherryExpressionBacterialPromoterT7 lac promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mClover LIC cloning vector (MBP-mClover)
Plasmid#86930PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mCloverDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialPromoterT7Available SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS mScx (CT#404)
Plasmid#13958DepositorInsertScleraxis (Scx Mouse)
ExpressionBacterialAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJF131B
Plasmid#113326PurposepTet-W108 scRNA.b2, pTet-RR2-sgRNADepositorInsertW108 scRNA_MS2 and sgRNA_RR2
UseCRISPRExpressionBacterialAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF5
Plasmid#37219DepositorInserteIF5
ExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97(QQ)
Plasmid#14667DepositorAvailable SinceApril 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPS2966
Plasmid#8925DepositorAvailable SinceAug. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046942
Plasmid#145757PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CLK3
Plasmid#38831PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ0285
Plasmid#11304DepositorInsertsmall heat shock protein (MJ_0285 M. jannaschii)
ExpressionBacterialAvailable SinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
CBLB
Plasmid#39056PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB3 AurA
Plasmid#68270Purposebacterial expression of human AurADepositorInsertAurA (AURKA Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationSee depositor comment belowPromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-T38M-750-luxAB-DAS+4
Plasmid#49520PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomallyDepositorInsertT38M-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL + STS
Plasmid#139790Purposeexpression of biosynthetic enzymes 4CL and STSDepositorInserts4CL
STS
ExpressionBacterialAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
ExpressionBacterialPromoterlacIqAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT106b
Plasmid#31392DepositorInsertred light inverter
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
SNX5-PX (26-170)
Plasmid#119086PurposeBacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
TagsSNAPExpressionBacterialAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Nedd4-C / 2523 JM15
Plasmid#13311DepositorAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-EPN-05
Plasmid#79920PurposeMyr4-12GS-I3-01-myc-Late1DepositorInsertEPN-05
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-10
Plasmid#79925PurposePalm4-12GS-I3-01-myc-Late1DepositorInsertEPN-10
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtipiTrc
Plasmid#53275PurposeContains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.DepositorAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-beta1,4 GT-1
Plasmid#11872DepositorInsertbeta 1,4 galactosyltransferase (B4GALT1 Human)
ExpressionBacterialAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
TagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaba
Plasmid#47620PurposeRecombinant protein productionDepositorAvailable SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-SELP0K
Plasmid#67007PurposeExpresses malE-SELP0K in E. coliDepositorInsertmalE-SELP0K
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only