31,059 results
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Plasmid#136611PurposeDox-inducible lentiviral vector expressing mouse Oct4DepositorInsertOct4 (Pou5f1 Mouse)
UseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046933
Plasmid#145592PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GB1-PRD(703-800)-Strep
Plasmid#141344PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-800)-Strep
TagsGB1-His6-TEV and Strep-tag (WSHPQFEK)ExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-V220A
Plasmid#47645PurposepAC-EsaR with a c659t mutation in esaRDepositorInsertesaR-V220A
UseSynthetic BiologyExpressionBacterialMutationChanged Valine 220 to AlaninePromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2
Plasmid#140670Purpose3-color stripeDepositorInsert3-color stripe
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_mCherry_GFP_++
Plasmid#187389Purposearabinose-inducible expression of GFP and mCherryDepositorInsertGFP, mCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPR
Plasmid#39161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertsepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
TagsHis TevExpressionBacterialMutationcodon-optimizedAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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NCOR2
Plasmid#40737PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNCOR2 (NCOR2 Human)
Tags6xHis TEVExpressionBacterialMutationResidues 615 to 685 of the SANT2 domain of Nuclea…Available SinceOct. 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS Survivin
Plasmid#19233DepositorInsertSurvivin (BIRC5 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK-GW
Plasmid#126556PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatibleDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2P1Egfp
Plasmid#90162Purposecontains lmo2 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Tre
Plasmid#123129PurposeBacterial expression plasmid for Tre with an N-terminal His-tag.DepositorInsertTre recombinase
Tags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
mOrange in pBSDONR P4r-P2
Plasmid#121529PurposeFor making C-terminal mOrange fusions using multisite Gateway cloningDepositorInsertmOrange
ExpressionBacterialAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4-GB1His6_AG
Plasmid#148792PurposeBacterial Expression of HsNot4DepositorInsertHsNot4 (CNOT4 Human)
ExpressionBacterialMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD395
Plasmid#163102PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmCherry_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy37
Plasmid#43998DepositorTypeEmpty backboneTagsHis6 and MBPExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSABAD505
Plasmid#45615DepositorInsertGFPN-NpuDnaE_intein-GFP_C
TagsGFP_C and GFP_NExpressionBacterialMutationstemmer GFP with superfolder mutations S30R and Y…PromoterpBADAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
CDC25C
Plasmid#39059PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_deltaLC
Plasmid#98664PurposeExpresses MBP-tagged hnRNPA2 without the LC domainDepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSPPc-Gm
Plasmid#116919PurposeGene constitutive expression from a Pc promoterDepositorTypeEmpty backboneExpressionBacterialPromoterPc promoter from Delftia acidovorans C17Available SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-APEX2
Plasmid#117751PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHis-hPim1
Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
TagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MACROD1
Plasmid#39041PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYTK071
Plasmid#65178PurposeEncodes ConR5 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR5
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
B5-4
Plasmid#111483PurposeTrp-tRNA synthetaseDepositorInsertTrp-tRNA synthetase
Tags5xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-PaIct
Plasmid#111293Purposebacterial expressionDepositorInsertsuccinyl-CoA:itaconate CoA transferase
TagsHis tagExpressionBacterialAvailable SinceJan. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro1GFP
Plasmid#82365PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro1GFP
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204CPromoterT7Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXC13(human)-pET
Plasmid#8679DepositorInsertHOXC13 (HOXC13 Human)
TagsHis and T7ExpressionBacterialMutationmakes protein by Coomassie blue stainingAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-GFP-Patronin CC 868–1457
Plasmid#59053Purposefor E. coli expression of Patronin CC truncationDepositorAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SETD3
Plasmid#32873PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/HisB (TIRSTD)-sfGFP
Plasmid#189674Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
TagsHis-T7-EKExpressionBacterialPromoterpBADAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-2
Plasmid#12994DepositorInsertTSP-2 (THBS2 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUB2 (full-length, aa 1-234)
Plasmid#61421PurposeExpresses human OTUB2 (full-length) in E. coli.DepositorInsertOTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
TagsHis6-GST-3CExpressionBacterialPromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC210
Plasmid#65950PurposeWeak Repressor plasmid for two-strain systemDepositorInsertCinI
TagsLAA taggedExpressionBacterialPromoterPrhl/lac-wAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1*
Plasmid#104000PurposePlasmid encoding mRFP1*DepositorInsertmRFP1*
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLAVIKEF...T…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
ExpressionBacterialMutationNAAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DST-1/1
Plasmid#91288PurposeProtein expression and purification of human SH3 domain construct DST-1/1DepositorInsertDST-1/1 (DST Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only