32,167 results
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Plasmid#129281PurposeThe vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellumDepositorTypeEmpty backboneExpressionBacterialPromoterClostridium thermocellum DSM1313 Cellobiose Phosp…Available SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only
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ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadR)-DadR
Plasmid#191636PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementationDepositorInsertDadR
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZFYVE9C-c009
Plasmid#53618Purposebacterial expression plasmidDepositorInsertZFYVE9C
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HS-Utrophin-GFP
Plasmid#105952Purposeheat shock inducible transgenesisDepositorAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP578
Plasmid#98808PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-GST
ExpressionBacterialMutationNoneAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP808
Plasmid#98812PurposeExpression VectorDepositorInsertpET28 MBP-HNLYGHS-GST
ExpressionBacterialMutationNoneAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
4QQ6
Plasmid#61946PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSMN1:JMC047-F11:C221089 (SMN1 Human)
Tags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pReporter_7
Plasmid#60567PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_7
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRIM33 (TRIM33B-c100)
Plasmid#98243PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTRIM33B (TRIM33 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00628:PHD 889-932, pfam00439:Bromodomain 963-…PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT937
Plasmid#68936PurposeRhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpRDepositorInsertInt12
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 CARD WT
Plasmid#164008PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARDDepositorAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
sfTq2-C1
Plasmid#117932PurposeSuperfolder mTurquoise2DepositorInsertsfTq2
ExpressionBacterialAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
TagsHRV3C cleavage site and StrepII-tag withExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 FNBP3 WW domain #1
Plasmid#104233PurposeBacterial expression of WW domain #1 from FNBP3DepositorInsertFNBP3 WW domain #1 (PRPF40A Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK563
Plasmid#110612PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoterDepositorInsertNanoluc
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbA5k-RFP
Plasmid#35282DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
Tags8xHisExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SMARCA2
Plasmid#73250PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbA7a-RFP
Plasmid#35316DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-kan
Plasmid#108703PurposeExpression of an His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsHis-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOPOII Pdx1 (DM#260)
Plasmid#15025DepositorInsertPdx1 (Pdx1 Mouse)
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneTagsHis6-hPDIb'a' tagExpressionBacterialAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(C)-SH2
Plasmid#46497DepositorInsertSHP-2(C) (PTPN11 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 101-222PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pUA66 PcspA GFP Linker
Plasmid#105604PurposeExpresses GFP under temperature sensitive promoter PcspADepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterPcspAAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC3-ISH
Plasmid#15854DepositorAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
inhibin-DsRed
Plasmid#156184PurposeExpression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.DepositorInsertsrainbow trout inhibin α promoter
rainbow trout β actin 3'UTR
ExpressionBacterialPromoterinhibinAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMR150
Plasmid#32292DepositorInsertgenomic methylation probe-minor satellite repeat
ExpressionBacterialAvailable SinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-11
Plasmid#134803PurposeIPTG inducible expression of S-TAGA-Evo1 qtRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT892
Plasmid#68933PurposeInt9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt9
ExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-CVm-PTEN
Plasmid#168032PurposeExpresses eCFP-PTEN(M265-N329)-mVenus in bacterial cellsDepositorInserteCFP-PTEN(M265-N329)-mVenus
TagsHisx6 and mycExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFAB3833
Plasmid#47839PurposeBIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.DepositorInsertpromoter 8 and BCD1
UseSynthetic BiologyExpressionBacterialPromotersee insertAvailable SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX7 Chromo
Plasmid#59695PurposeContains histone modification interacting domain (CBX7 Chromo)DepositorAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30-hDlg PDZ2
Plasmid#21692DepositorInserthDlg/SAP97 PDZ2 domain (DLG1 Human)
TagsHis6ExpressionBacterialMutationhuman hDlg/SAP97 PDZ2 domain (residues 318-405)Available SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TUBG1
Plasmid#171967PurposepGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion proteinDepositorInsertTUBG1 (TUBG1 Human)
TagsGSTExpressionBacterialMutationR275H (Please see depositor comments)Available SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
VGFR1_HUMAN_D0
Plasmid#79743PurposeThis plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVGFR1
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only