32,218 results

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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STK24_HUMAN_D0

Plasmid

#79742

Purpose

This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK24 (STK24 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT3

Plasmid

#53559

Purpose

bacterial expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pBS-KSTbx2.8.2

Plasmid

#42626

Depositor

Virginia Papaioannou

Article

Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

Insert

Tbx2 (Tbx2 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
pET21b-RL015A

Plasmid

#42134

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

sfGFP-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK4-EE

Plasmid

#47577

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK4-EE (MAP2K4 Human)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
DARS_pNIC-Bio3

Plasmid

#153048

Purpose

Expression construct for producing Avi tagged Glycyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

DARS (DARS1 Human)

Tags

Avi and His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCool-hDDB1-BPB

Plasmid

#48639

Purpose

Expresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)

Depositor

Ning Zheng

Article

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

hDDB1-BPB (DDB1 Human)

Expression

Bacterial

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#131372

Purpose

expression of MBP:PGL-1 in C. elegans

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

pgl-1 (pgl-1 Nematode)

Expression

Bacterial

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 D321N

Plasmid

#53202

Purpose

Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
T4 Lysozyme S38D L99A M102Q N144D

Plasmid

#18477

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Mutation

S38D L99A M102Q N144D

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
GABPA_pet28a

Plasmid

#131640

Purpose

Bacterial expression of ETS gene GABPA

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

GABPA (GABPA Human)

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKCyR5

Plasmid

#149463

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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env8 selectivity (b')

Plasmid

#99837

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (b')

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046806

Plasmid

#145746

Purpose

Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTKDP-cat

Plasmid

#71321

Purpose

Donor plasmid - chloramphenicol resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGST-PPARdelta

Plasmid

#16548

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta (PPARD Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-249

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pIcaRA-I1

Plasmid

#74682

Purpose

IcaRA-I1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSI1-IcaRA-PIcaR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTarget-RFP-GFP operon

Plasmid

#192279

Purpose

RFP GFP fusion protein expressed under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP GFP

Expression

Bacterial

Promoter

PlacIq

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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tIVR

Plasmid

#122663

Purpose

Express tIVR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tIVR

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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6MIW

Plasmid

#127291

Purpose

Bacterial expression for structure determination. May not contain entire coding region of gene

Depositor

Cheryl Arrowsmith

Article

Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

Insert

HUWE1 (HUWE1 Human)

Tags

6xHis-TEV (MHHHHHHSSGRENLYFQG)

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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SPI1_pet28a

Plasmid

#131653

Purpose

Bacterial expression of ETS gene SPI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPI1 (SPI1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGB

Plasmid

#49203

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

H6-FGB

Tags

His tag + Other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

None

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-cxcr4b whim-gfp

Plasmid

#41089

Depositor

Anna Huttenlocher

Article

Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

Insert

CXCR4b-WHIM (cxcr4b Zebrafish)

Tags

GFP

Expression

Bacterial

Mutation

deleted last 19 amino acids of cxcr4b

Promoter

mpx

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCR2.1_HBB_N

Plasmid

#87847

Purpose

pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

Depositor

Marina Kleanthous

Article

Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

Insert

Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b_NOSER

Plasmid

#124244

Purpose

Bacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.

Depositor

Attila Fehér

Type

Empty backbone

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPhlF-P2

Plasmid

#74686

Purpose

PhlF-P2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSP2-PhlF-PPhlF-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Tags

His and S

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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