31,059 results
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Plasmid#183474PurposeThis plasmid can be used to prepare Fluc mRNA by In Vitro TranscriptionDepositorInsertFirefly luciferase
UseLuciferaseExpressionBacterialPromoterT7Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBJ6
Plasmid#167135PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterKanamycin promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-6X
Plasmid#27118DepositorInsertMS2 binding sites (6)
ExpressionBacterialAvailable SinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21b Hot-PETase
Plasmid#225761PurposeExpresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tagDepositorInsertHot-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
USP5
Plasmid#25299PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWM_12x601_30bpLinker
Plasmid#157789PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_30bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I DCARDS
Plasmid#210828Purposeprotein expression in ecoliDepositorInserthRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
ExpressionBacterialMutationNAAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-HA2
Plasmid#165991PurposeSpyCatcher-fused influenza HA ministem with removable HisTagDepositorInsertSpyCatcher-HA2
TagsFoldon, HisTag, and SpyCatcherExpressionBacterialPromoterT7Available SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET11a-p52(1-398)
Plasmid#44733DepositorAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
Tags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612AAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSUN2
Plasmid#188059PurposeBacterial expression plasmid for human NSUN2; for activity testingDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYC01
Plasmid#224586PurposeIPTG-inducible phage φX174 lysis gene EDepositorInsertphage φX174 lysis gene E (phiX174p08 )
UseSynthetic BiologyExpressionBacterialPromoterPA1lacO-1Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHYHD1
Plasmid#38854PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
Tags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_mCherry_Bba_B0015
Plasmid#107581PurposeB. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialPromoterpdh promoter Bacillus megaterium DSM319Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-RGVG-M6
Plasmid#70172PurposeExpresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNADepositorInsertRGVG-M6 T7 RNA polymerase
TagsHisExpressionBacterialPromoterT5/LacAvailable SinceDec. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
prhaBAD-anti-mouse-Nb-Hia5
Plasmid#239626PurposeRhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferaseDepositorInsertHia5
Tags6HIS, MBP, TEV, and anti-Mouse NanobodyExpressionBacterialMutationCodon optimized for bacterial expressionPromoterrhaBADAvailable SinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKM201
Plasmid#13078DepositorInsertPtac-gam-bet-exo operon
ExpressionBacterialAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
TagsHisExpressionBacterialPromoterT7 promoterAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRF-TetR
Plasmid#49374DepositorInsertTetR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_Δpvc10
Plasmid#198448PurposeProduces PVCs containing truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_Δpvc10
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
M57 pPET28a-TEV-full-length human beta catenin
Plasmid#17198DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialPromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Km
Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
lox2272 Neo* lox2272
Plasmid#168769Purposelox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
Plasmid#198753PurposeCre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activityDepositorInsertEF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
UseLentiviralExpressionBacterial and MammalianMutationSee depositor comments belowPromoterEF1aAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p1A
Plasmid#162692PurposeExpresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-BRD4ΔN
Plasmid#200968Purposeinduction of phase-separated condensate mediated by BRD4dN-IDRDepositorInsertBRD4dN
ExpressionBacterial and MammalianAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF-GFPplus
Plasmid#172718PurposeExpression of a fast folding GFP variant GFP plusDepositorInsertGFP plus
ExpressionBacterialAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC020 - LseCas13a from Listeria seeligeri
Plasmid#91910PurposeExpresses LseCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLseCas13a and guide expression cassette
ExpressionBacterialAvailable SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Taq
Plasmid#102793PurposeExpresses Taq DNA polymerase under WT T7 promoterDepositorInsertTaq DNA polymerase
ExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
ExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only