29,776 results

1. pRSETB/mStayGold

   Plasmid

   #212017

   Purpose

   mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDsgRNA-p15

   Plasmid

   #62656

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-KG-D4H*-mCherry

   Plasmid

   #134604

   Purpose

   fluorescent cholesterol-binding probe

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   D4H*
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   Y415A and A463W

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTn Donor

   Plasmid

   #236211

   Purpose

   Arabinose-inducible Tn5 InducTn-seq plasmid

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   None
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCre

   Plasmid

   #236212

   Purpose

   Arabinose-inducible Cre recombinase

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgRNA-bacteria

   Plasmid

   #44251

   Purpose

   Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pATetO 6XHis-Phusion

   Plasmid

   #206210

   Purpose

   Inducible expression of DNA polymerase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

   Insert

   Phusion DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pA-His6-Tn5

    Plasmid

    #171871

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Protein A and hyperactive Tn5 transposase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest-527

    Plasmid

    #11518

    Purpose

    Gateway destination vector for bacterial expression; His tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL2299A

    Plasmid

    #222105

    Purpose

    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

    Depositor

    Kan Wang

    Article

    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

    Insert

    virA
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    virA promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NW30

    Plasmid

    #133444

    Purpose

    Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW30
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-nanoHER2-SpyTag003

    Plasmid

    #216312

    Purpose

    Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    nanoHER2-SpyTag003
    

    Use

    Tags

    His6 tag and SpyTag003

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Sfp

    Plasmid

    #159617

    Purpose

    Expression plasmid for Sfp phosphopantetheinyl transferase

    Depositor

    Jun Yin, Christopher T Walsh

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    Sfp
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-AspS

    Plasmid

    #159618

    Purpose

    Expression plasmid for AcpS phosphopantetheinyl transferase

    Depositor

    Jun Yin, Christopher T Walsh

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    AcpS
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNC02

    Plasmid

    #233644

    Purpose

    For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    sfGFP with c-terminal VitelloTagB
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEcCas-2.0

    Plasmid

    #190697

    Purpose

    Constitutive expressing of Cas9 and inducible expression of lambda Red

    Depositor

    Sheng Yang

    Article

    Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

    Insert

    Cas9 and Lamda Red system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge